Kokcidióza patří mezi nejčastější onemocnění králíků v době odstavu a je jedním z limitujících faktorů odchovu králíků. Mezi patogenní kokcidie řadíme u králíků druhy parazitující ve střevech (E. magna, E. irresidua, E. intestinalis, E. flavescens, E. piriformis, a E. media) a jaterní kokcidie (E. stiedae). Stupeň infekce je závislý na druhu Eimerie, množství pozřených oocyst, věku a imunitním stavu hostitele. Diagnostika je založena na flotačním vyšetření, případně na přesném určení druhu pomocí molekulárněbiologických metod. Terapie spočívá v aplikaci antikokcidik (sulfonamidů, toltrazurilu) ve vodě nebo podaných přímo do dutiny ústní. Prevence onemocnění spočívá v dobrých zoohygienických podmínkách, správně provedeném odstavu, krmné dávce a jejím složení (v obsahu vlákniny, škrobů a bílkovin), snížení stresových faktorů a v případě velkochovů nebo malochovů v podání peletovaného krmiva s obsahem kokcidiostatik.
Coccidiosis is one of the most common diseases of rabbits at the time of weaning and is one of the limiting factors in rabbit breeding. Pathogenic coccidia in rabbits includes species parasitizing in the intestines (E. magna, E. irresidua, E. intestinalis, E. flavescens, E. piriformis, and E. media) and liver (E. stiedae). The severity of infection depends on the species of Eimeria, the number of ingested oocysts, age, and immune status of the host. Diagnostics is based on a flotation examination, or the exact determination of the species using molecular biological methods. Therapy consists of the oral administration of anticoccidials (sulphonamides, toltrazuril) in water or directly into the oral cavity. Disease prevention consists of optimal zoohygiene, properly performed weaning, feed ration and its composition (fiber, starch, and protein content), reduction of stress factors, and, in the case of large-scale or small-scale farms, in the form of pelleted diets containing coccidiostatics.
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- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.
- MeSH
- infračervené záření MeSH
- lidé MeSH
- preparace izolovaného srdce * metody MeSH
- termoregulace MeSH
- zahřívání metody přístrojové vybavení MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
Encephalitozoon cuniculi is an obligate intracellular pathogen that has a wide host distribution, but primarily affects rabbits. The aim of this study was to characterize both the cell-mediated and the antibody response in rabbits after experimental infection using 2 different infection routes: oral and ocular. SPF rabbits were infected with low (10³ spores) and high (10⁷ spores) infection doses. Monitored parameters included clinical signs, detection of spores in urine, antibody response detected with ELISA, and cell-mediated immunity detected by antigen-driven lymphocyte proliferation. At week 13 post-infection, half of the rabbits in each group were suppressed by intramuscular administration of dexamethasone. At week 18 post-infection, animals were euthanized. Clinical signs were mild with exacerbation after immunosuppression. Spores in urine and antigen-specific cell-mediated immunity were detected from weeks 5 and 4 post-infection, respectively. Specific IgM was detected 1 week after infection, and IgG antibodies followed 1 week later in rabbits infected with the high dose. Immunological responses were dose dependent. The authors can conclude that both oral and ocular experimental infection with E. cuniculi resulted in an immune response of the infected animals. Rabbits could be used as an experimental model for the study of ocular microsporidiosis.
- MeSH
- aktivace lymfocytů MeSH
- buněčná imunita MeSH
- Encephalitozoon cuniculi imunologie patogenita MeSH
- encephalitozoonóza imunologie parazitologie patologie MeSH
- imunoglobulin G krev MeSH
- imunoglobulin M krev MeSH
- králíci MeSH
- modely nemocí na zvířatech MeSH
- nemoci úst imunologie parazitologie patologie MeSH
- oční infekce imunologie parazitologie patologie MeSH
- outbrední kmeny zvířat MeSH
- protilátky protozoální krev MeSH
- tvorba protilátek MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
