MUC13, a transmembrane mucin glycoprotein, is overexpressed in colorectal cancer (CRC), however, its regulation and functions are not fully understood. It has been shown that MUC13 protects colonic epithelial cells from apoptosis. Therefore, studying MUC13 and MUC13-regulated pathways may reveal promising therapeutic approaches for CRC treatment. Growing evidence suggests that microRNAs (miRs) are involved in the development and progression of CRC. In the present study, the MUC13-miR-4647 axis was addressed in association with survival of patients. miR-4647 is predicted in silico to bind to the MUC13 gene and was analyzed by RT-qPCR in 187 tumors and their adjacent non-malignant mucosa of patients with CRC. The impact of previously mentioned genes on survival and migration abilities of cancer cells was validated in vitro. Significantly upregulated MUC13 (P=0.02) in was observed tumor tissues compared with non-malignant adjacent mucosa, while miR-4647 (P=0.05) showed an opposite trend. Higher expression levels of MUC13 (log-rank P=0.05) were associated with worse patient's survival. The ectopic overexpression of studied miR resulted in decreased migratory abilities and worse survival of cells. Attenuated MUC13 expression levels confirmed the suppression of colony forming of CRC cells. In summary, the present data suggested the essential role of MUC13-miR-4647 in patients' survival, and this axis may serve as a novel therapeutic target. It is anticipated MUC13 may hold significant potential in the screening, diagnosis and treatment of CRC.
- Publikační typ
- časopisecké články MeSH
This article compares two important pathophysiological states, Kawasaki disease, and multisystem inflammatory syndrome, in children associated with COVID-19 (MIS-C). Both occur predominantly in children, have a temporal association with an infectious agent, and are associated with immune-system alteration and systemic inflammation under certain circumstances. The two share common pathophysiology, including enhancement of interleukin-1 neutrophils, activation of the inflammasome, pyroptosis, or NETosis. Moreover, the clinical presentation of the diseases overlaps. However, they are indeed two separate diseases, proven by the differences in the epidemiological and etiological aspects and the pathophysiological processes involved in the development and frequency of some clinical signs. This article highlights potentially exciting areas that have not yet been studied in detail, which could help better understand the development of these diseases.
- MeSH
- COVID-19 * MeSH
- dítě MeSH
- Kawasakiho syndrom * diagnóza MeSH
- lidé MeSH
- syndrom systémové zánětlivé reakce MeSH
- zánět MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
1. elektronické vydání 1 online zdroj (90 stran)
Na téma krev a hematopoéza je možné se dívat z různých úhlů pohledu. Publikace obsahuje popisnou část i bohatou obrazovou dokumentaci jak mikrofotografií jednotlivých vývojových stadií krevních elementů a samotných krevních elementů, tak i jejich vybraných patologických odchylek. Krevní elementy jsou zachyceny v periferní krvi a v kostní dřeni. Zahrnuty jsou také elektronogramy a originální schémata vzniklá na jejich podkladě. Začleněn je popis krevních skupin a jejich vývoje. V neposlední řadě je publikace doplněna o vybrané metody zkoumání krve. Věříme, že se tak pre- i postgraduálním studentům medicíny a souvisejících disciplín dostane důležitá pomůcka nejen pro studium preklinických, ale i klinických oborů.
- Klíčová slova
- Hematologie,
- MeSH
- cytologické techniky MeSH
- hematologické testy MeSH
- hematopoéza MeSH
- histologické techniky MeSH
- kostní dřeň MeSH
- krev MeSH
- NLK Obory
- hematologie a transfuzní lékařství
Učební texty Univerzity Karlovy
First edition 89 stran : ilustrace, tabulky ; 23 cm
The textbook focuses on blood, blood elements and cytological, histological and haematological methods.
Liver cirrhosis is associated with impairment of cardiovascular function including alterations of the heart innervation, humoral and nervous dysregulation, changes in systemic circulation and electrophysiological abnormalities. Choline acetyltransferase (ChAT), enzyme forming acetylcholine, tyrosine hydroxylase (TH), and dopamine-β-hydroxylase (DBH), enzymes participating in noradrenaline synthesis, are responsible for the production of classical neurotransmitters, and atrial natriuretic peptide (ANP) is produced by cardiomyocytes. The aim of this study was to evaluate the influence of experimentally induced hepatic dysfunction on the expression of proANP, ChAT, TH, and DBH in the heart. Hepatic dysfunction was induced by application of thioacetamide (TAA) or by ligation of bile duct. Biochemical parameters of hepatic injury and levels of peroxidation in the liver and heart were measured. Liver enzymes measured in the plasma were significantly elevated. Cardiac level of peroxidation was increased in operated but not TAA group animals. In the left atrium of operated rats, the expression of TH and DBH was lower, while expression of ChAT remained unchanged. In TAA group, no significant differences in the expression of the genes compared to controls were observed. Liver injury induced by ligation leads to an imbalance in the intracardiac innervation, which might impair nervous control of the heart.
- MeSH
- akční potenciály MeSH
- atriální natriuretický faktor krev metabolismus MeSH
- dopamin-beta-hydroxylasa krev metabolismus MeSH
- jaterní cirhóza enzymologie metabolismus patofyziologie MeSH
- játra enzymologie patofyziologie MeSH
- krysa rodu rattus MeSH
- myokard enzymologie metabolismus MeSH
- oxidační stres MeSH
- peroxidace lipidů MeSH
- regulace genové exprese * MeSH
- srdce fyziologie MeSH
- svalová kontrakce MeSH
- tyrosin-3-monooxygenasa krev metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
