PURPOSE: The DTL fibre electrode is commonly used to record the electric potentials elicited by stimulation of the retina. Two positions are commonly used: it is placed either on the cornea along the lower lid or in the conjunctival fornix. The PERG and OPs have previously been examined and compared under both conditions. The aim of this study was to examine the ERG, flicker response and on-off responses with differing electrode positions. METHODS: Before recruitment, all subjects underwent an ophthalmological examination. We enrolled 13 normal control subjects into the study aged 13-64 years, all with a visual acuity of ≥1.0. We recorded scotopic and photopic ERGs, flicker and on-off responses, for both electrode positions. On the first day, one eye had the electrode placed on the cornea along the lower lid and the other eye had it positioned in the conjunctival sac. On a second day, the recordings were repeated with the alternative electrode placements. RESULTS: ERG, on-off and flicker responses were all smaller by between 20 and 25% when the DTL electrode was positioned in the conjunctival sac, compared to when it was positioned on the cornea, as did the scatter in the data points. This indicates that there is no advantage clinically for one or the other placement. CONCLUSIONS: Our results confirm other reports examining the effect of electrode position on electrophysiological potentials. When recording with the DTL electrode, it is important to ensure that it is placed at the same position in repeat recordings or in multicentre trials and that it is stable and does not move during recording.

• MeSH
• dospělí MeSH
• elektrofyziologie metody   MeSH
• elektroretinografie metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikroelektrody * MeSH
• mladiství MeSH
• mladý dospělý MeSH
• retina fyziologie   MeSH
• rohovka fyziologie   MeSH
• studie případů a kontrol MeSH
• světelná stimulace metody   MeSH
• zraková ostrost MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

PURPOSE: Several methods are routinely used in the clinic to diagnose and monitor diseases of inner retinal function. In this study, we compare four such methods in patients with diabetes and glaucoma, to determine correlations between their results and to determine which method is most sensitive for detecting disease. METHODS: Twenty control subjects, 12 patients with early glaucoma and eight patients with diabetes mellitus, were enrolled in the study. All underwent four examinations: transient pattern electroretinogram (PERG), multifocal pattern electroretinogram (mfPERG), chromatic contrast threshold measurements (protan and tritan), and blue-on-yellow short-wavelength automated perimetry (SWAP). RESULTS: For the total cohort of 40 subjects, the results show a significant correlation between the amplitudes of the PERG and those of the mfPERG, as well as between the tritan contrast thresholds and the SWAP MD. Furthermore, ROC analyses reveal that colour contrast thresholds could significantly distinguish between the patient and the control group. Glaucoma patients alone could also be distinguished. CONCLUSIONS: We conclude that the methods compared in this study show correlations between their results if they are testing same pathway or underling cells, and that the colour contrast threshold is the most sensitive method to detect early functional deficits in diabetic and glaucoma patients.

• MeSH
• čípky retiny fyziologie   MeSH
• citlivost na kontrast fyziologie   MeSH
• diabetes mellitus 1. typu diagnóza   patofyziologie   MeSH
• diabetická retinopatie diagnóza   patofyziologie   MeSH
• dospělí MeSH
• elektrofyziologické jevy MeSH
• elektrofyziologie metody   MeSH
• elektroretinografie metody   MeSH
• glaukom diagnóza   patofyziologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• poruchy barevného vidění diagnóza   patofyziologie   MeSH
• ROC křivka MeSH
• senioři MeSH
• senzorické prahy MeSH
• testy zrakového pole MeSH
• zraková pole fyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH