The process of colonization of Ascaris lumbricoides eggs by the fungus Verticillium chlamydosporium was studied by scanning electron microscopy. The preparations were made by fractionation of egg suspension exposed to the fungus for four days and frozen in liquid nitrogen according to Stĕrba and Milácek (1986). Ovicidal fungus forms an abundant ramifying mycelial network in the area between the eggs. However, egg-shells are penetrated only by some hyphae without any penetration organs produced (simple hyphal penetration). In a liquid medium, after penetration, hyphae inside the eggs rapidly grow among inner structures of egg-shells and on the surface of developing larvae. In the next phase, hyphae colonize the developing larva. The eggs attacked by this fungus remain morphologically unchanged for a long time except the sites of penetration. Verticillium chlamydosporium is a fungus with unique ovicidal properties. It colonizes the eggs of Ascaris lumbricoides at all stages of embryo development and also attacks larvae inside the eggs.
The formation of chitin-protein layer in Ascaris lumbricoides egg-shells was studied using the method of chitin structures visualization by means of derivatives of stilbene-disulfonic acid (Blankophor, Bayer, FRG). Intensive formation of chitin structures in A. lumbricoides egg-shell occurred only in fertilized females in a very short portion of uterus from the site of connection of the oviduct with the uterus up to 25-30 mm distally, i.e. in the section forming about one fifth or less than one sixth of length of adult female uterus. Already 3 mm from the beginning of uterus there was a thin layer on the surface of the fertilized eggs which contained chitin detectable by Blankophor visualization, whereas the chitin-protein layer on the shells could not be demonstrated histologically. The thickness of this chitin-protein complex increased distally. At 25 mm from the beginning of uterus the chitin layer was already complete and its thickness did not increase any more.
- MeSH
- Ascaris anatomie a histologie MeSH
- chitin analýza biosyntéza MeSH
- fluorescenční barviva MeSH
- fluorescenční mikroskopie MeSH
- histocytochemie MeSH
- organické látky MeSH
- ovum ultrastruktura MeSH
- proteiny červů analýza biosyntéza MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
105 s.
- Klíčová slova
- španělština - slovníky terminologické - texty učební VŠ,
- Konspekt
- Všeobecná příručková díla
- NLK Obory
- všeobecná příručková díla a encyklopedie
1. vyd. 73 s. : il. ; 28 cm
- Konspekt
- Buněčná biologie. Cytologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- parazitologie
- NLK Publikační typ
- učebnice vysokých škol
2. vyd. 161 s. : obr., tab.
- Konspekt
- Buněčná biologie. Cytologie
- NLK Obory
- parazitologie
- biologie
- NLK Publikační typ
- učebnice vysokých škol
1. vyd. 53 s. : il. ; 29 cm
- MeSH
- parazitologie MeSH
- Konspekt
- Buněčná biologie. Cytologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- parazitologie
- NLK Publikační typ
- učebnice vysokých škol
Učební texty Univerzity Palackého
76 s. : il.
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- environmentální vědy
- NLK Publikační typ
- učebnice vysokých škol
Under the light microscope the chitin-protein layer of egg-shells in ascarids appears to be a regular, hyaline and nonstructural layer of 1.5 to 2.00 microns in thickness. The outer uterine layer is usually removed during the preparation. The lipid (ascaroside) layer covers the inner surface of the chitinous layer and seems to be irregularly undulated and regularly thick over the whole surface, with the thickness up to 1 micron. In electron micrographs the fibrous structure of the lipid layer is not evident as a rule. This is probably due to washing the lipids away from this layer during the dehydration of deeper layers of egg-shells that are imperfectly fixed with glutaraldehyde. A very low permeability of the egg-shells is typical of geohelminth eggs. The layer lipid shows a distinct lamellate structure only after a prolonged fixation with osmium at higher temperature. This is supported by the studies using the method of freeze-fracturing.
- MeSH
- Ascaris ultrastruktura MeSH
- chitin MeSH
- elektronová mikroskopie MeSH
- lipidy MeSH
- mrazové lámání MeSH
- ovum ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
