Recurrent aphthous stomatitis (RAS) is the most common disease of the oral mucosa, and it has been recently associated with bacterial and fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, and at an unaffected site, compared with healed mucosa in RAS patients and healthy controls. We sampled microbes from five distinct sites in the oral cavity. The one site with the most pronounced differences in microbial alpha and beta diversity between RAS patients and healthy controls was the lower labial mucosa. Detailed analysis of this particular oral site revealed strict association of the genus Selenomonas with healed mucosa of RAS patients, whereas the class Clostridia and genera Lachnoanaerobaculum, Cardiobacterium, Leptotrichia, and Fusobacterium were associated with the presence of an active ulcer. Furthermore, active ulcers were dominated by Malassezia, which were negatively correlated with Streptococcus and Haemophilus and positively correlated with Porphyromonas species. In addition, RAS patients showed increased serum levels of IgG against Mogibacteriumtimidum compared with healthy controls. Our study demonstrates that the composition of bacteria and fungi colonizing healthy oral mucosa is changed in active RAS ulcers, and that this alteration persists to some extent even after the ulcer is healed.

• Publication type
• Journal Article MeSH

• Publication type
• Meeting Abstract MeSH

Práce shrnuje současné poznatky o problematice gloso- a stomato- dynií. Pod pojem dynie nezahrnujeme jen primárně nepříjemné pocity, ale všechny stavy, kde na „první pohled“ není jasná etiologie potíží v dutině ústní. Jedná se o onemocnění multifaktoriální, jehož jediným společným znakem je nepříjemný pocit v dutině ústní. Je velmi obtížné stanovit nějakou klasifikaci či konkrétní jednotný postup vyšetření a terapie. Cílem práce bylo zaměřit se na rozlišení primární dynie od sekundární. K vyloučení nesprávně stanovené diagnózy z příčin, které by se mohly spolupodílet na vzniku a rozvoji dynie, bude vždy zapotřebí mezioborové spolupráce.

This article tries to summarize current knowledge about the matters of glosso- and stomato- dynia. The term of glossodynia does not include only primarily unpleasant feeling but also all states where „at first sight“ there is not clear cause of the complaints in oral cavity. It is a multifactorial disease whose only common sign is the unpleasant feeling in oral cavity and, therefore, it is very difficult to set some classification or particular uniform procedure of examination and treatment. The aim is to concentrate on the diagnoses which are necessary to distinguish from primary dynia. To eliminate wrongly determined diagnosis which could participate in formation and development of dynia, interdisciplinary cooperation will always be needed.

• MeSH
• Toothache etiology   complications   MeSH
• Diagnosis, Differential MeSH
• Gingivitis complications   MeSH
• Glossalgia * diagnosis   etiology   MeSH
• Humans MeSH
• Mouth Diseases * diagnosis   etiology   MeSH
• Facial Pain diagnosis   etiology   MeSH
• Burning Mouth Syndrome * diagnosis   etiology   classification   MeSH
• Mouth Mucosa pathology   drug effects   MeSH
• Oral Manifestations MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH

• MeSH
• Humans MeSH
• Oral Hygiene MeSH
• Pathology, Oral etiology   MeSH
• Signs and Symptoms MeSH
• Oral Manifestations MeSH
• Check Tag
• Humans MeSH

• MeSH
• Humans MeSH
• Oral Hygiene MeSH
• Pathology, Oral MeSH
• Oral Manifestations MeSH
• Check Tag
• Humans MeSH