Mössbauer spectrometry is a progressive method of analysis of materials which is widely used for the study of various molecular and biomolecular systems, as well as various other objects and materials containing Mössbauer nuclides. This work is focused on applications of Mössbauer spectroscopy in the area of studying iron in biological tissues. The results of the published works show that iron in this case is present in the oxidation states Fe II and Fe III , and constitutes a part of molecules, such as ferritin, ferrihydrite, hematite, and haemosiderin. Low-temperature measurements of some types of biological tissues confirmed the presence of paramagnetic and superparamagnetic components.

• Klíčová slova
• Mössbauerova spektrometrie,
• MeSH
• chemické techniky analytické metody   MeSH
• fluorescenční spektrometrie metody   MeSH
• játra chemie   MeSH
• lidé MeSH
• mozek - chemie MeSH
• slezina chemie   MeSH
• tkáně chemie   MeSH
• výzkum MeSH
• železo * analýza   krev   MeSH
• Check Tag
• lidé MeSH

Iron is an essential element for fundamental cell functions and a catalyst for chemical reactions. Three samples extracted from the human spleen were investigated by scanning (SEM) and transmission electron microscopy (TEM), Mössbauer spectrometry (MS), and SQUID magnetometry. The sample with diagnosis of hemosiderosis (H) differs from that referring to hereditary spherocytosis and the reference sample. SEM reveals iron-rich micrometer-sized aggregate of various structures-tiny fibrils in hereditary spherocytosis sample and no fibrils in hemochromatosis. Hematite and magnetite particles from 2 to 6 μm in TEM with diffraction in all samples were shown. The SQUID magnetometry shows different amount of diamagnetic, paramagnetic and ferrimagnetic structures in the tissues. The MS results indicate contribution of ferromagnetically split sextets for all investigated samples. Their occurrence indicates that at least part of the sample is magnetically ordered below the critical temperature. The iron accumulation process is different in hereditary spherocytosis and hemosiderosis. This fact may be the reason of different iron crystallization.

• MeSH
• dědičná sférocytóza metabolismus   patologie   MeSH
• hemosideróza metabolismus   patologie   MeSH
• krystalizace MeSH
• lidé MeSH
• oxid železnato-železitý chemie   MeSH
• pitva MeSH
• slezina chemie   metabolismus   patologie   ultrastruktura   MeSH
• spektroskopie Mossbauerova MeSH
• transmisní elektronová mikroskopie MeSH
• železité sloučeniny chemie   metabolismus   MeSH
• železo chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH