Hypercholesterolemia (HCH) is characterized by an increase of the total- and LDL-cholesterol in serum. In hypercholesterolemia, generally recognized as a risk factor of atherogenesis, oxidative stress and oxidatively modified LDL play a crucial role. In our study, children with elevated total cholesterol (above 4.5 mmol/l) were included. Parameters of lipid profile, lipophilic vitamins and antioxidants (retinol, ?-tocopherol, ?-tocopherol, xantophyll, lycophen and ß-carotene) and markers of oxidative damage to lipids (lipoperoxides and 8-isoprostanes) were evaluated. We found that children with hypercholesterolemia have significantly increased parameters of lipid profile and these are gender dependent only in HDL-cholesterol (1.27 ± 0.10 mmol/l in boys vs. 1.53 ± 0.07 mmol/l in girls; p<0.05) and TAG (1.63 ± 0.31 mmol/l in boys vs. 1.08 ± 0.09 mmol/l in girls; p<0.05). In addition, children with HCH have decreased total antioxidant capacity of serum (TEAC) (about 19.64%, p<0.05) and increased lipoperoxides (LP) (about 45.73%, p<0.001). We have revealed statistically significant correlations between parameters of lipid profile and lipophilic vitamins and antioxidants, as well as between markers of oxidative stress: positive correlation between LP and 8-iso (r=0.353, n=33, p<0.05) and negative correlations between these parameters and TEAC (r= –0.377, n=33, p<0.05 for LP and r= –0.379, n=33, p<0.05 for 8-iso). In conclusion, we confirmed relation between hypercholesterolemia and oxidative stress and effect of gender on these processes already in childhood. Since the atherosclerotic process begins in childhood before clinical symptoms, early detection of hypercholesterolemia and oxidative stress is important in later atherosclerosis prevention.

• MeSH
• antioxidancia analýza   MeSH
• dinoprost analogy a deriváty   krev   MeSH
• dítě MeSH
• financování organizované MeSH
• hypercholesterolemie metabolismus   MeSH
• lidé MeSH
• lipidové peroxidy krev   MeSH
• lipidy krev   MeSH
• mladiství MeSH
• oxidační stres MeSH
• pohlavní dimorfismus MeSH
• vitaminy krev   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Paraoxonase 1 (PON1) seems to have a relevant role in detoxifying processes and in atherosclerosis. The aim of this study was to determine PON1 activity, the total antioxidant capacity, as well as entire lipid profile in children for screening of possible risk of atherosclerosis development. Serum PON1 arylesterase/paraoxonase activities were determined spectrophotometrically. The total antioxidant capacity of the serum was measured by TEAC method. Parameters of lipid profile were analyzed by routine laboratory methods. It has been shown that PON1 arylesterase/ paraoxonase activities were very similar to values found in adults. In children, no significant correlation between PON1 arylesterase activity and HDL was observed. PON1 paraoxonase activity correlated only with atherogenic index. PON1 arylesterase activity was significantly higher in girls than in boys. The antioxidant capacity was inversely related to the body mass index. In this study, PON1 activity was determined in healthy children aged 11 to 12 years and we found a similarity in PON1 activities of children and adults. Moreover, the results of our study support the hypothesis that higher body weight of children may contribute to a greater risk for development of atherosclerosis in which oxidative stress plays a role.

• MeSH
• antioxidancia MeSH
• aryldialkylfosfatasa chemie   izolace a purifikace   MeSH
• ateroskleróza etiologie   prevence a kontrola   MeSH
• dítě MeSH
• finanční podpora výzkumu jako téma MeSH
• interpretace statistických dat MeSH
• lidé MeSH
• lipidové peroxidy izolace a purifikace   škodlivé účinky   MeSH
• lipoproteiny HDL izolace a purifikace   škodlivé účinky   MeSH
• rizikové faktory MeSH
• tělesná hmotnost MeSH
• Check Tag
• dítě MeSH
• lidé MeSH

• MeSH
• algoritmy MeSH
• diazepam aplikace a dávkování   terapeutické užití   MeSH
• dítě MeSH
• febrilní křeče etiologie   klasifikace   terapie   MeSH
• horečka farmakoterapie   terapie   MeSH
• lidé MeSH
• rizikové faktory MeSH
• rodiče výchova   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• přehledy MeSH