Mycobacterium avium subsp. hominissuis (Mah) infection was diagnosed in 5 captive bongo antelopes (Tragelaphus eurycerus) originating from a collection in a zoological garden. The animals suffered from emaciation. Postmortem examination revealed nodular lesions in the lungs of all 5 examined animals. Acid-fast bacilli were observed in the lungs of 4 animals. Culture and polymerase chain reaction identification based on IS901 negativity and IS1245 positivity confirmed Mah infection in the lungs of all 5 antelopes. In 3 animals, Mah was also isolated from other organs (liver, spleen, and kidney). Molecular analysis of these isolates using IS1245 restriction fragment length polymorphism and/or mycobacterial interspersed repetitive units-variable number tandem repeat revealed that the studied antelopes were infected by 1 identical genotype. Furthermore, in 2 antelopes, other genotypes were also detected. This shows the possibility of either genetic modifications occurring during infection or polyclonal infection. Culture examination of environmental samples from the enclosures holding the bongos revealed Mah in mulch bark, peat, and soil. Genotyping of these environmental isolates determined several genotypes with 1 dominant genotype that was identical to the dominant genotype detected in antelopes.
- MeSH
- antilopy mikrobiologie MeSH
- DNA bakterií chemie genetika MeSH
- fatální výsledek MeSH
- feces mikrobiologie MeSH
- genotyp MeSH
- incidence MeSH
- Mycobacterium avium genetika izolace a purifikace MeSH
- plicní nemoci epidemiologie mikrobiologie veterinární MeSH
- polymerázová řetězová reakce veterinární MeSH
- půdní mikrobiologie MeSH
- transpozibilní elementy DNA genetika MeSH
- tuberkulóza epidemiologie mikrobiologie veterinární MeSH
- zvířata v ZOO MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
From Mycobacterium avium species Mycobacterium avium subsp. paratuberculosis (n=961), Mycobacterium a. avium (n=677), Mycobacterium a. silvaticum (n=5), and Mycobacterium a. hominissuis (n=1566) were examined, and from Mycobacterium tuberculosis complex M. tuberculosis (n=2), Mycobacterium bovis (n=13), M. bovis BCG (n=4), and Mycobacterium caprae (n=10) were examined. From other mycobacterial species Mycobacterium intracellulare (n=60) and atypical mycobacteria (n=256) including Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium scrofulaceum, Mycobacterium gastri and other species of conditionally pathogenic mycobacteria were analysed. The internal standard molecules corresponding to insertion sequences IS900, IS901, IS1245, and flanking region (FR300) of IS901 were produced by PCR of alfalfa genome segment and inserted into plasmid vector. The resulting recombinant plasmid molecules were used as internal standards in coamplification with a total of 4729 mycobacterial collection strains and field isolates between 1996 and 2003. The size differences between amplicons obtained from IS900 (258 bp), IS901 (1108 bp), IS1245 (427 bp), and FR300 (300 bp) and from corresponding internal standard molecules ISIS900 (591 bp), ISIS901 (1 336 bp), ISIS1245 (583 bp), and IS901 flanking region of 300 bp ISFR300 (488 bp), respectively, allowed easy discrimination. The internal amplicons were visible by naked aye on agarose gel when 10(1), 10(3), 10(2), and 10(2) molecules for ISIS900, ISIS901, ISIS1245, and ISFR300 were used in the PCR, respectively, when no bacterial DNA was added to the reaction. The system was tested to define the amount of internal standards that could be used in the PCR without affecting the amplification of the specific segment. Non-specific amplifications were observed in M. fortuitum with IS1245 PCR and mixed infections with M. a. avium and M. a. hominissuis from pigs and cattle were found. PCR results of typing were compared with serotyping and Accu-Probes analyses in selected field isolates.
- MeSH
- DNA bakterií genetika chemie MeSH
- financování organizované MeSH
- Mycobacterium genetika izolace a purifikace klasifikace MeSH
- mykobakteriózy diagnóza mikrobiologie veterinární MeSH
- nemoci prasat diagnóza mikrobiologie MeSH
- nemoci skotu diagnóza mikrobiologie MeSH
- polymerázová řetězová reakce metody veterinární MeSH
- prasata MeSH
- referenční standardy MeSH
- sérotypizace MeSH
- skot MeSH
- transpozibilní elementy DNA genetika MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
