Sarcomas are a heterogeneous group of mesenchymal tumours, with a great variability in their clinical behaviour. While our knowledge of sarcoma initiation has advanced rapidly in recent years, relatively little is known about mechanisms of sarcoma progression. JUN-murine fibrosarcoma progression series consists of four sarcoma cell lines, JUN-1, JUN-2, JUN-2fos-3, and JUN-3. JUN-1 and -2 were established from a single tumour initiated in a H2K/v-jun transgenic mouse, JUN-3 originates from a different tumour in the same animal, and JUN-2fos-3 results from a targeted in vitro transformation of the JUN-2 cell line. The JUN-1, -2, and -3 cell lines represent a linear progression from the least transformed JUN-2 to the most transformed JUN-3, with regard to all the transformation characteristics studied, while the JUN-2fos-3 cell line exhibits a unique transformation mode, with little deregulation of cell growth and proliferation, but pronounced motility and invasiveness. The invasive sarcoma sublines JUN-2fos-3 and JUN-3 show complex metabolic profiles, with activation of both mitochondrial oxidative phosphorylation and glycolysis and a significant increase in spared respiratory capacity. The specific transcriptomic profile of invasive sublines features very complex biological relationships across the identified genes and proteins, with accentuated autocrine control of motility and angiogenesis. Pharmacologic inhibition of one of the autocrine motility factors identified, Ccl8, significantly diminished both motility and invasiveness of the highly transformed fibrosarcoma cell. This progression series could be greatly valuable for deciphering crucial aspects of sarcoma progression and defining new prognostic markers and potential therapeutic targets.
- Publikační typ
- časopisecké články MeSH
Alternatives and their teaching are an essential part of the curricula at the Faculty of Medicine. Dynamic screen-based video recordings are the most important type of alternative models employed for teaching purposes. Currently, the majority of teaching materials for this purpose are based on PowerPoint presentations, which are very popular because of their high versatility and visual impact. Furthermore, current developments in the field of image capturing devices and software enable the use of digitised video streams, tailored precisely to the specific situation. Here, we demonstrate that with reasonable financial resources, it is possible to prepare video sequences and to introduce them into the PowerPoint presentation, thereby shaping the teaching process according to individual students' needs and specificities.
- MeSH
- apoptóza MeSH
- buněčné jádro patologie MeSH
- HL-60 buňky patologie MeSH
- lidé MeSH
- počítačové zpracování obrazu metody MeSH
- Check Tag
- lidé MeSH
