Chronic wounds and their associated bacterial infections are major issues in modern health care systems. Therefore, antimicrobial resistance (AMR), treatment costs, and number of disability-adjusted life-years have gained more interest. Recently, photodynamic therapy emerged as an effective approach against resistant and naïve bacterial strains with a low probability of creating AMR. In this study, needleless electrospinning was used to produce an indocyanine green (ICG) loaded poly(d,l-lactide) nanofibrous mesh as a photoresponsive wound dressing. The non-woven mesh had a homogeneous nanofibrous structure and showed long-term hydrolytic stability at different pH values. The antimicrobial activity was tested against several bacterial strains, namely Staphylococcus saprophyticus subsp. bovis, Escherichia coli DH5 alpha, and Staphylococcus aureus subsp. aureus. Upon irradiation with a laser of a specific wavelength (λ = 810 nm), the bacterial viability was significantly reduced by 99.978% (3.66 log10), 99.699% (2.52 log10), and 99.977% (3.64 log10), respectively. The nanofibrous mesh showed good biocompatibility, which was confirmed by the proliferation of mouse fibroblasts (L929) on the surface and into deeper parts of the mesh. Furthermore, a favorable proangiogenic effect was observed in ovo using the chorioallantoic membrane assay. In general, it can be concluded that ICG loaded nanofibers as an innovative wound dressing represent a promising strategy against chronic wounds associated with skin infections.
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčné linie MeSH
- chorioalantoická membrána krevní zásobení účinky léků MeSH
- Escherichia coli účinky léků růst a vývoj MeSH
- fibroblasty cytologie účinky léků MeSH
- fotochemoterapie MeSH
- indokyanová zeleň chemie farmakologie MeSH
- mikrobiální viabilita účinky léků MeSH
- myši MeSH
- nanovlákna MeSH
- obvazy MeSH
- polyestery chemie MeSH
- proliferace buněk účinky léků MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- Staphylococcus saprophyticus účinky léků růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
