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6 záznamů v Medvik Filtry

Článek

Co-delivering of oleuropein and lentisk oil in phospholipid vesicles as an effective approach to modulate oxidative stress, cytokine secretion and promote skin regeneration

Sklenarova, Renata
Autor Sklenarova, Renata Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic
Allaw, Mohamad
Autor Allaw, Mohamad Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy
Perra, Matteo
Autor Perra, Matteo Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy
Castangia, Ines
Autor Castangia, Ines Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy
Frankova, Jana
Autor Frankova, Jana Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic
Luis Pedraz, Josè
Autor Luis Pedraz, Josè NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Letizia Manca, Maria
Autor Letizia Manca, Maria Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy. Electronic address: mlmanca@unica.it
Manconi, Maria
Autor Manconi, Maria Dept. Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy

European journal of pharmaceutics and biopharmaceutics. 2023 ; 185 (-) : 126-136. [pub] 20230305

Eur J Pharm Biopharm
ISSN 1873-3441
Medvik
Zdroj

In this work oleuropein and lentisk oil have been co-loaded in different phospholipid vesicles (i.e., liposomes, transfersomes, hyalurosomes and hyalutransfersomes), to obtain a formulation capable of both inhibiting the production of different markers connected with inflammation and oxidative stress and promoting the skin repair. Liposomes were prepared using a mixture of phospholipids, oleuropein and lentisk oil. Tween 80, sodium hyaluronate or their combination have been added to the mixture to obtain transfersomes, hyalurosomes and hyalutransfersomes. Size, polydispersity index, surface charge and stability on storage was evaluated. The biocompatibility, anti-inflammatory activity and wound healing effect were tested using normal human dermal fibroblasts. Vesicles were small (mean diameter ∼ 130 nm) and homogeneously dispersed (polydispersity index ∼ 0.14), highly negatively charged (zeta potential 02053-64 mV) and capable of loading 20 mg/mL of oleuropein and 75 mg/mL of lentisk oil. The freeze-drying of dispersions with a cryoprotectant permitted to improve their stability on storage. The co-loading of oleuropein and lentisk oil in vesicles inhibited the overproduction of inflammatory markers, especially MMP-1 and IL-6, counteracted the oxidative stress induced in cells using hydrogen peroxide, and promoted the healing of a wounded area performed in vitro in a cell monolayer of fibroblasts. The proposed co-loading of oleuropein and lentisk oil in natural-based phospholipid vesicles may hold promising therapeutic value especially for the treatment of a wide variety of skin disorders.

Článek

Collagen as a Biomaterial for Skin and Corneal Wound Healing

Journal of functional biomaterials. 2022 ; 13 (4) : . [pub] 20221116

J Funct Biomater
ISSN 2079-4983
Medvik
Zdroj

The cornea and the skin are two organs that form the outer barrier of the human body. When either is injured (e.g., from surgery, physical trauma, or chemical burns), wound healing is initiated to restore integrity. Many cells are activated during wound healing. In particular, fibroblasts that are stimulated often transition into repair fibroblasts or myofibroblasts that synthesize extracellular matrix (ECM) components into the wound area. Control of wound ECM deposition is critical, as a disorganized ECM can block restoration of function. One of the most abundant structural proteins in the mammalian ECM is collagen. Collagen type I is the main component in connective tissues. It can be readily obtained and purified, and short analogs have also been developed for tissue engineering applications, including modulating the wound healing response. This review discusses the effect of several current collagen implants on the stimulation of corneal and skin wound healing. These range from collagen sponges and hydrogels to films and membranes.

Článek

Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro

Journal of Applied Biomedicine. 2021 ; 19 (3) : 149-158.

ISSN 1214-021X
Medvik
Zdroj

Myricetin (MYR) and dihydromyricetin (DHM) are classified as natural flavonoids. Both substances are known for their anti-inflammatory and antioxidant properties. In this study, an in vitro model of inflammation was demonstrated on monolayers of scratched fibroblasts or keratinocytes exposed to LPS from Pseudomonas aeruginosa for six hours. MYR and DHM were subsequently applied to the cells for 24 hours at sub toxic concentrations (5-15 μM). Inflammatory parameters were analysed in collected cell medium and lysate after the incubation period using the Enzyme-Linked ImmuneSorbent Assay (ELISA) and Western blot. Both flavonoids inhibit the production of pro-inflammatory cytokines (IL-6, IL-8) in LPS-stimulated skin cells as well as the decreased level of MMP-1 in fibroblasts. However, the application of MYR and DHM dose dependently increased the level of MMP-1 in keratinocytes. In our experiments, we focused on the anti-glycation activity of MYR and DHM, where the higher concentration of MYR seems to be more effective.

Článek

Impact of family level factors on alcohol drinking in primary school children

Central European Journal of Public Health. 2013 ; 21 (4) : 202-206.

Cent. Eur. J. Public Health
ISSN 1210-7778
Medvik
Zdroj

OBJECTIVE: The aim of our study was to identify and analyze selected factors influencing alcohol use among primary school children aged 8 to 15 years in the academic year 2009/10. METHODS: The sample consisted of children from 28 primary schools. Data of this cross-sectional study were collected using questionnaires. Chi-Square test was used to test differences in proportions of observed phenomena between boys and girls. Multiple logistic regression analysis was performed to identify the influence of selected factors on the variable "child's alcohol drinking per week". RESULTS: The sample consisted of 2,494 respondents (52% of boys, 48% of girls). In the study group 78% of all respondents (95% CI=76-80) drank alcohol infrequently (less than once a week) or did not drink alcohol at all, and 22% of respondents (95% Cl=20-24) drank alcohol at least once a week. More boys than girls considered alcoholic beverages such as beer, wine and spirit as quite easily available. We performed the model of multivariate logistic regression analysis using the independent variables: age of respondents, gender of respondents, mother's alcohol drinking, father's alcohol drinking, sibling's alcohol drinking, parental rules, parental control, and mental support from parents (if their children have problems) to identify their effect on the dependent variable - child's alcohol drinking per week. We found out that mother's alcohol drinking and a lack of mental support from parents did not have a statistically significant influence on child's alcohol drinking per week. CONCLUSION: The results of our study point to the fact that it is necessary to focus on the prevention of alcohol drinking in general and among school children in particular. We recommend greater control of the sale of alcoholic beverages, so as to prevent the purchase by people under 18 years of age.