In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine.

• MeSH
• dextrany metabolismus   MeSH
• fluorescenční spektrometrie MeSH
• intracelulární prostor metabolismus   MeSH
• lidé MeSH
• magnetické nanočástice chemie   MeSH
• mezenchymální kmenové buňky cytologie   metabolismus   MeSH
• molekulární sondy chemie   metabolismus   MeSH
• molekulární zobrazování metody   MeSH
• rhodaminy chemie   MeSH
• tuková tkáň cytologie   MeSH
• viabilita buněk MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Rehabilitace se skládá z léčby zaměstnáním (prací), z léčebného tělocviku a z fyzikální terapie. Všechny tři složky tvoří nerozlučný celek, neboť je nutno pečovat o celou osobnost nemocného a nejen o poraněnou část těla.|Důležité je také udržet u pacienta zájem o práci

• MeSH
• rehabilitace MeSH