The distribution for voriconazole and fluconazole susceptibility was determined by Etest and disk diffusion test in 143 clinical isolates. The majority of the strains of Aspergillus spp., Candida krusei, C. inconspicua, C norvegensis and Saccharomyces cerevisiae displayed resistance or decreased susceptibility to fluconazole in contrast to voriconazole. The absolute categorical agreement for voriconazole and fluconazole susceptibility results by the disk method and Etest was 90.5 and 74.8 % respectively. The error rate bounding analysis showed only 0.7 % of false susceptible results ( very major error) with voriconazole, but 2.8 % with fluconazole. Fluconazole can be used as a surrogate factor to predict voriconazole susceptibility but with lower reliability for susceptible-dose dependent and resistance category, especially in Candida glabrata isolates. The results of the disk method were not substantially influenced by the composition of media (Mueller-Hinton agar vs antimycotic Sensitivity Test agar), even if with the latter the results had fewer tendencies to produce false susceptibility of C.glabrata isolates to both of the triazole drugs. Disk test as well as Etest were shown to represent suitable methods for routine evaluation of susceptibility of clinical isolates of pathogenic fungi, including aspergilli, to fluconazole and voriconazole.
- MeSH
- antifungální látky farmakologie MeSH
- diskové difúzní antimikrobiální testy metody MeSH
- flukonazol farmakologie MeSH
- fungální léková rezistence MeSH
- houby izolace a purifikace účinky léků MeSH
- kultivační média chemie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mykózy farmakoterapie mikrobiologie MeSH
- pyrimidiny farmakologie MeSH
- triazoly farmakologie MeSH
- Check Tag
- lidé MeSH
- MeSH
- amfotericin B aplikace a dávkování toxicita MeSH
- antifungální látky chemie izolace a purifikace MeSH
- Candida albicans izolace a purifikace růst a vývoj účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- flucytosin aplikace a dávkování toxicita MeSH
- gama-butyrolakton analogy a deriváty aplikace a dávkování toxicita MeSH
- guanidiny aplikace a dávkování toxicita MeSH
- kandidóza chronická mukokutánní diagnóza MeSH
- lidé MeSH
- salicylanilidy aplikace a dávkování toxicita MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
The mechanism of action of the antifungal agent 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans was investigated by flow cytometry, using propidium iodide, DiBAC4(3), and FUN-1 as the fluorescent dyes. A related but less active agent, together with amphotericin B and fluconazole, was tested in parallel for comparison of the results. The incrustoporine derivative was found to have a potent fungicidal activity on C. albicans, resulting in damage of cell membrane.
