Fifteen lactic acid bacterial strains were isolated from blood cultures from 15 different patients in the Faculty Hospital in Brno, Czech Republic. All strains were identified using biochemical tests and repetitive PCR using the (GTG)5 primer. Doubtful identification results were confirmed by whole-cell protein analysis. The strains were assigned to the genera Lactobacillus (eight strains representing seven species), Leuconostoc (six strains representing four species) and Weissella (one strain). Antibiotic susceptibility testing was performed using the E-test and revealed high-level resistance to cotrimoxazol, metronidazole, vancomycin and teicoplanin, but nearly all strains were susceptible to erythromycin, clindamycin, ampicillin and penicillin.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Bacterial Proteins analysis   MeSH
• Bacteremia microbiology   MeSH
• Child MeSH
• DNA, Bacterial genetics   MeSH
• Adult MeSH
• Financing, Organized MeSH
• Gram-Positive Bacterial Infections microbiology   MeSH
• Gram-Positive Bacteria physiology   genetics   isolation & purification   MeSH
• Infant MeSH
• Lactobacillus physiology   genetics   isolation & purification   MeSH
• Leuconostoc physiology   genetics   isolation & purification   MeSH
• Middle Aged MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Adolescent MeSH
• Hospitals, University MeSH
• Polymerase Chain Reaction MeSH
• Child, Preschool MeSH
• Repetitive Sequences, Nucleic Acid genetics   MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Bacterial Typing Techniques MeSH
• Check Tag
• Child MeSH
• Adult MeSH
• Infant MeSH
• Middle Aged MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Child, Preschool MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH
• Geographicals
• Czech Republic MeSH

Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA-DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99% similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of alpha-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213(T) (=LMG 22723(T)) as the type strain.

• MeSH
• Bacterial Proteins analysis   MeSH
• Chaperonin 60 genetics   MeSH
• DNA, Bacterial analysis   MeSH
• Financing, Organized MeSH
• Phylogeny MeSH
• Gastrointestinal Tract microbiology   MeSH
• Genes, rRNA MeSH
• Nucleic Acid Hybridization MeSH
• Molecular Sequence Data MeSH
• Ribotyping MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Saimiri microbiology   MeSH
• Sequence Analysis, DNA MeSH
• Staphylococcus physiology   genetics   isolation & purification   classification   MeSH
• Bacterial Typing Techniques MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Geographicals
• South America MeSH

Two enterococcal strains LMG 16607(T) and LMG 16612 originating from sea water were analysed in a polyphasic taxonomic study. Both strains, assigned as Enterococcus sp. in the BCCM/LMG culture collection, possessed analogous protein profiles, but these were different from all other enterococcal species. 16S rRNA gene sequence analysis of one strain showed the highest similarity, 96.9-96.1%, with its closest phylogenetic neighbours Enterococcus saccharolyticus, Enterococcus sulfureus, Enterococcus saccharominimus and Enterococcus italicus. Further genomic analysis by (GTG)(5)-PCR fingerprinting and sequence analysis of the housekeeping gene phenylalanyl-tRNA synthase (pheS) and distinct biochemical features confirmed that the two strains represent a novel enterococcal species for which the name Enterococcus aquimarinus sp. nov. is proposed. The type strain is LMG 16607(T) (=CCM 7283(T)).

• MeSH
• DNA, Bacterial analysis   MeSH
• DNA Fingerprinting methods   MeSH
• Enterococcus genetics   chemistry   isolation & purification   classification   MeSH
• Phenotype MeSH
• Phenylalanine-tRNA Ligase genetics   MeSH
• Financing, Organized MeSH
• Phylogeny MeSH
• Genes, rRNA MeSH
• Molecular Sequence Data MeSH
• Seawater microbiology   MeSH
• DNA, Ribosomal analysis   MeSH
• RNA, Ribosomal, 16S MeSH
• Sequence Analysis, DNA MeSH
• Bacterial Typing Techniques MeSH