This study is a thorough examination of the effects of the DNA polymerase inhibitor aphidicolin on the nuclear cycle and cell cycle progression characteristics, as well as their reversibility, in Giardia intestinalis. Giardia trophozoites are arrested in the G1/S-junction after aphidicolin treatment according to their DNA content. However, cell growth continues and trophozoites arrested with aphidicolin resemble cells in the G2 phase and trophozoites in ageing cultures. Extensive treatment with aphidicolin causes side effects and we detected positive signals for phosphorylated histone H2A, which, in mammalian cells, is involved in a signalling pathway triggered as a reaction to double stranded DNA breaks. These results suggest that aphidicolin causes dissociation of the nuclear and cytoplasmic cycles, a phenomenon that has also been described for other inhibitors in mammalian cell lines. Thus, if aphidicolin is used for synchronization of Giardia trophozoites, this fact must be accounted for, and treatment with aphidicolin must be minimal. Copyright 2009 Elsevier Inc. All rights reserved.
- MeSH
- afidikolin farmakologie MeSH
- bromodeoxyuridin metabolismus MeSH
- buněčný cyklus účinky léků MeSH
- časové faktory MeSH
- cyklin B analýza MeSH
- DNA-dependentní DNA-polymerasy MeSH
- fluorescenční protilátková technika MeSH
- fosforylace účinky léků MeSH
- Giardia lamblia cytologie genetika účinky léků MeSH
- histony metabolismus MeSH
- inhibitory enzymů farmakologie MeSH
- inhibitory syntézy nukleových kyselin MeSH
- mitotický index MeSH
- poškození DNA účinky léků MeSH
- protozoální DNA biosyntéza účinky léků MeSH
- průtoková cytometrie MeSH
- replikace DNA účinky léků MeSH
- trofozoiti cytologie účinky léků MeSH
- Publikační typ
- práce podpořená grantem MeSH
