ETHNOPHARMACOLOGICAL RELEVANCE: Different plants are used for the treatment of various ailments and Acorus calamus L. is one such plant found in Western Himalaya. Rhizome of this plants has ethnomedicinal significance, as its rhizome is used for curing fever, pain and inflammation. An attempt has been made to alter the phytochemicals and increase its antioxidant property in a sustainable way with the help of mycorrhizal inoculation. AIM OF THE STUDY: Study of mycorrhizal (Funneliformis mosseae) impact on the biological activities and phytochemical profile of A. calamus L. rhizome and in silico studies of phytochemicals for their anti-inflammatory property. MATERIALS AND METHODS: F. mosseae was mass multiplied by single spore culture and then A. calamus rhizomes were inoculated with it. Antioxidant potential of rhizome extract was observed by DPPH and FRAP assays and the phytochemical profiling was done with GC-MS analysis. For observing antimicrobial activity disc diffusion method was employed. Dominant phytochemicals α-asarone and monolinolein TMS were chosen for molecular docking studies against four receptors (4COX, 2AZ5, 5I1B, 1ALU). RESULTS: There was increase in antioxidant activity of rhizome extract after mycorrhizal inoculation. However, no change in antimicrobial activity was observed in the plant after mycorrhizal inoculation. The comparison in phytochemicals was observed by GC-MS analysis which showed qualitative and quantitative variation in biochemical content in plants. The phytochemical, α-asarone and monolinolein TMS showed highest docking score and least binding energy against 1ALU and 4COX respectively for anti-inflammatory activity. CONCLUSION: Medicinal plants are potential source of antioxidants which can be increased by mycorrhizal inoculation without addition of chemical fertilizers and also results in altering the phytochemical composition.
- MeSH
- antiflogistika izolace a purifikace farmakologie MeSH
- antiinfekční látky izolace a purifikace farmakologie MeSH
- antioxidancia izolace a purifikace farmakologie MeSH
- diskové difúzní antimikrobiální testy MeSH
- fytonutrienty izolace a purifikace farmakologie MeSH
- houby fyziologie MeSH
- mykorhiza fyziologie MeSH
- oddenek MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- počítačová simulace MeSH
- puškvorec chemie mikrobiologie MeSH
- rostlinné extrakty farmakologie MeSH
- simulace molekulového dockingu MeSH
- tradiční lékařství MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie MeSH
In the present investigation Hibiscus rosa-sinensis (petals), Acorus calamus (rhizome), Moringa oleifera (leaves) and Cucurbita maxima (petals) were screened for their efficacy against osteosarcoma with elucidating a mechanism of their anticancer potentiality. The methanolic plant extracts revealed the presence of all major phytochemicals with quantitative analysis of flavonoids (98.15 ± 2.02 to 12.34 ± 0.57 mg of RUE/g) and total phenolics (26.40 ± 0.11 to 8.54 ± 0.10 mg of GAE/g). The antioxidant activity was assessed by standard DPPH, H2O2 scavenging, NO scavenging assays. The hemolysis, hemagglutination, and erythrocyte aggregation assays unveiled their compatibility with blood components. As most of the opportunistic microbes infect subsequently immunocompromised patients, the antimicrobial activity of the plant extracts showed a zone of inhibition (in mm) against nosocomial strains of Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Vibrio cholerae having MIC between 12.5–50 μg/ml. Through MTT assay the IC50 value was calculated against MG 63 osteosarcoma with detailed studies on DNA fragmentation and chromatin condensation, revealing apoptosis being their primary mode of anticancer effect. Further the migration and colony forming assays supported the anticancer potentials of the methanolic plant extracts. The cell cycle analysis revealed that A. calamus and M. oleifera extracts were capable of arresting the growth of MG 63 osteosarcoma cells.
- MeSH
- antioxidancia MeSH
- antitumorózní látky fytogenní * farmakologie chemie MeSH
- Cucurbita chemie MeSH
- fytoterapie MeSH
- Hibiscus chemie MeSH
- léčivé rostliny MeSH
- léky antitumorózní - screeningové testy MeSH
- mikrobiální testy citlivosti MeSH
- Moringa chemie MeSH
- nádorové buňky kultivované účinky léků MeSH
- osteosarkom farmakoterapie MeSH
- puškvorec chemie MeSH
- rostlinné extrakty MeSH
- techniky in vitro MeSH
- Klíčová slova
- puškvorec obecný,
- MeSH
- fytoterapie MeSH
- léčivé rostliny MeSH
- lidé MeSH
- puškvorec MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- populární práce MeSH
