In the present investigation Hibiscus rosa-sinensis (petals), Acorus calamus (rhizome), Moringa oleifera (leaves) and Cucurbita maxima (petals) were screened for their efficacy against osteosarcoma with elucidating a mechanism of their anticancer potentiality. The methanolic plant extracts revealed the presence of all major phytochemicals with quantitative analysis of flavonoids (98.15 ± 2.02 to 12.34 ± 0.57 mg of RUE/g) and total phenolics (26.40 ± 0.11 to 8.54 ± 0.10 mg of GAE/g). The antioxidant activity was assessed by standard DPPH, H2O2 scavenging, NO scavenging assays. The hemolysis, hemagglutination, and erythrocyte aggregation assays unveiled their compatibility with blood components. As most of the opportunistic microbes infect subsequently immunocompromised patients, the antimicrobial activity of the plant extracts showed a zone of inhibition (in mm) against nosocomial strains of Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Vibrio cholerae having MIC between 12.5–50 μg/ml. Through MTT assay the IC50 value was calculated against MG 63 osteosarcoma with detailed studies on DNA fragmentation and chromatin condensation, revealing apoptosis being their primary mode of anticancer effect. Further the migration and colony forming assays supported the anticancer potentials of the methanolic plant extracts. The cell cycle analysis revealed that A. calamus and M. oleifera extracts were capable of arresting the growth of MG 63 osteosarcoma cells.
- MeSH
- antioxidancia MeSH
- antitumorózní látky fytogenní * farmakologie chemie MeSH
- Cucurbita chemie MeSH
- fytoterapie MeSH
- Hibiscus chemie MeSH
- léčivé rostliny MeSH
- léky antitumorózní - screeningové testy MeSH
- mikrobiální testy citlivosti MeSH
- Moringa chemie MeSH
- nádorové buňky kultivované účinky léků MeSH
- osteosarkom farmakoterapie MeSH
- puškvorec chemie MeSH
- rostlinné extrakty MeSH
- techniky in vitro MeSH
We investigated the antidiabetic effect of Moringa olifeira Lam. in a diet-induced obesity (DIO) mouse model. Six mice were randomly selected as normal controls. Moringa olifeira Lam. leaf extract at a dose of 200, 400 or 600 mg/kg body weight, glibenclamide (Glib) at the dose of 10 mg/kg (positive control) and distilled water at 10 ml/kg (control group) were administered orally by gastric intubation, and each group consisted of six mice. Insulinsensitive tissues (liver, skeletal muscle) were collected to investigate antidiabetic effects and examine the plant's molecular mechanisms. Moringa olifeira Lam. leaf extract prevented weight gain. It also reduced blood glucose in DIO mice. Glib and Moringa olifeira Lam. leaf extract, 400 mg/kg, treatments restored insulin levels towards normal values (P < 0.05 versus diabetic control group). Western immunoblot analysis of different tissues, collected at the end of the study, demonstrated that Moringa olifeira Lam. stimulated activation of the insulin-dependent Akt pathway and increased the protein content of Glut 4 in skeletal muscle. The improvement of hepatic steatosis observed in DIO-treated mice was associated with a decrease in the hepatic content of SREBP-1, a transcription factor involved in de novo lipogenesis. The hepatic PPARα protein content in the plant extract- treated mice remained significantly higher than those of the control group (P < 0.05). In conclusion, this study provides the first evidence for direct action of Moringa olifeira Lam. on pancreatic β-cells, enhancing glucose-stimulated insulin secretion. This correlated with hypoglycaemic effects in diabetic mice associated with restored levels of plasma insulin.
- MeSH
- beta-buňky účinky léků metabolismus MeSH
- dieta škodlivé účinky MeSH
- experimentální diabetes mellitus farmakoterapie metabolismus MeSH
- hypoglykemika terapeutické užití MeSH
- inzulin metabolismus MeSH
- Moringa chemie MeSH
- myši MeSH
- obezita farmakoterapie etiologie MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- rostlinné extrakty terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Moringa oleifera is a plant whose fruits, roots and leaves have been advocated for traditional medicinal uses. The physicochemical analysis shows that Moringa oleifera contains more dietary polyunsaturated fatty acids (PUFA) than saturated fatty acids (SFA). The consumption of an experimental diet enriched with Moringa oleifera extracts lowered blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats as compared to rats fed an unsupplemented control diet. Anti-CD3-stimulated T cell proliferation was diminished in both strains of rats fed the Moringa oleifera. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. Studies of platelets from patients with primary hypertension and from SHR support the notion that the concentration of intracellular free calcium [Ca(2+)](i) is modified in both clinical and experimental hypertension. We observed that the basal, [Ca(2+)](i) was lower in T cells of SHR than in those of WKY rats fed the control diet. Feeding the diet with Moringa oleifera extracts to WKY rats did not alter basal [Ca(2+)](i) in T cells but increased basal [Ca(2+)](i) in SHR. Our study clearly demonstrated that Moringa oleifera exerts antihypertensive effects by inhibiting the secretion of IL-2 and modulates T cell calcium signaling in hypertensive rats.
- MeSH
- antihypertenziva izolace a purifikace farmakologie terapeutické užití MeSH
- hypertenze farmakoterapie imunologie patofyziologie MeSH
- krysa rodu rattus MeSH
- listy rostlin MeSH
- Moringa oleifera * MeSH
- potkani inbrední SHR MeSH
- potkani inbrední WKY MeSH
- rostlinné extrakty izolace a purifikace farmakologie terapeutické užití MeSH
- T-lymfocyty účinky léků fyziologie MeSH
- tělesná hmotnost účinky léků fyziologie MeSH
- vápníková signalizace účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Antimicrobial activity of crude seed extract of Moringa oleifera was investigated by thin layer chromatography bioassay against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Cladosporium cladosporioides, and Penicillium sclerotigenum; most of them were prominently inhibited by an isolate with R(F) 0.92-0.96. Characterization and identification of the extract revealed the occurrence of three bioactive compounds: 4-(?-L-rhamnopyranosyloxy)benzyl isothiocyanate, methyl N-4-(?-L-rhamnopyranosyloxy) benzyl carbamate (both known compounds), and 4-(ß-D-glucopyranosyl-1›4-?-L-rhamnopyranosyloxy)-benzyl thiocarboxamide, existence of which in any Moringa spp. or plant is reported for the first time. The UV spectrum of the novel compound showed maximum absorption at 273 and 225 nm in MeOH while the IR spectrum revealed several characteristic bands at 3100, 2900, 1700, 1500, 1300, 1100 and 1000 cm(-1). The (1)H-NMR showed signals at 1.2 and 3.77 ppm and the (13)C-NMR presented signals at 155, 122, 91.7 and 98.4 ppm. All the compounds at 5 mg/L had very high bactericidal activity against some of test pathogens even at contact period 1-2 h. 4-(ß-D-Glucopyranosyl-1›4-?-L-rhamnopyranosyloxy)benzyl thiocarboxamide was the most potent, with 99.2 % inhibition toward Shigella dysenteriae and 100 % toward Bacillus cereus, E. coli and Salmonella typhi within 4 h of contact.
- MeSH
- antiinfekční látky farmakologie MeSH
- Bacteria klasifikace účinky léků MeSH
- biotest MeSH
- chromatografie na tenké vrstvě metody MeSH
- glukosidy farmakologie MeSH
- glukosinoláty farmakologie chemie MeSH
- houby klasifikace účinky léků MeSH
- isothiokyanatany farmakologie chemie MeSH
- magnetická rezonanční spektroskopie MeSH
- mikrobiální testy citlivosti metody MeSH
- Moringa oleifera chemie MeSH
- počet mikrobiálních kolonií MeSH
- rostlinné extrakty farmakologie chemie MeSH
- semena rostlinná chemie MeSH
