Moringa oleifera is a plant whose fruits, roots and leaves have been advocated for traditional medicinal uses. The physicochemical analysis shows that Moringa oleifera contains more dietary polyunsaturated fatty acids (PUFA) than saturated fatty acids (SFA). The consumption of an experimental diet enriched with Moringa oleifera extracts lowered blood pressure in spontaneously hypertensive rats (SHR), but not in normotensive Wistar-Kyoto (WKY) rats as compared to rats fed an unsupplemented control diet. Anti-CD3-stimulated T cell proliferation was diminished in both strains of rats fed the Moringa oleifera. The experimental diet lowered secretion of interleukin-2 in SHR, but not in WKY rats compared with rats fed the control diet. Studies of platelets from patients with primary hypertension and from SHR support the notion that the concentration of intracellular free calcium [Ca(2+)](i) is modified in both clinical and experimental hypertension. We observed that the basal, [Ca(2+)](i) was lower in T cells of SHR than in those of WKY rats fed the control diet. Feeding the diet with Moringa oleifera extracts to WKY rats did not alter basal [Ca(2+)](i) in T cells but increased basal [Ca(2+)](i) in SHR. Our study clearly demonstrated that Moringa oleifera exerts antihypertensive effects by inhibiting the secretion of IL-2 and modulates T cell calcium signaling in hypertensive rats.
- MeSH
- antihypertenziva izolace a purifikace farmakologie terapeutické užití MeSH
- hypertenze farmakoterapie imunologie patofyziologie MeSH
- krysa rodu rattus MeSH
- listy rostlin MeSH
- Moringa oleifera * MeSH
- potkani inbrední SHR MeSH
- potkani inbrední WKY MeSH
- rostlinné extrakty izolace a purifikace farmakologie terapeutické užití MeSH
- T-lymfocyty účinky léků fyziologie MeSH
- tělesná hmotnost účinky léků fyziologie MeSH
- vápníková signalizace účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Antimicrobial activity of crude seed extract of Moringa oleifera was investigated by thin layer chromatography bioassay against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Cladosporium cladosporioides, and Penicillium sclerotigenum; most of them were prominently inhibited by an isolate with R(F) 0.92-0.96. Characterization and identification of the extract revealed the occurrence of three bioactive compounds: 4-(?-L-rhamnopyranosyloxy)benzyl isothiocyanate, methyl N-4-(?-L-rhamnopyranosyloxy) benzyl carbamate (both known compounds), and 4-(ß-D-glucopyranosyl-1›4-?-L-rhamnopyranosyloxy)-benzyl thiocarboxamide, existence of which in any Moringa spp. or plant is reported for the first time. The UV spectrum of the novel compound showed maximum absorption at 273 and 225 nm in MeOH while the IR spectrum revealed several characteristic bands at 3100, 2900, 1700, 1500, 1300, 1100 and 1000 cm(-1). The (1)H-NMR showed signals at 1.2 and 3.77 ppm and the (13)C-NMR presented signals at 155, 122, 91.7 and 98.4 ppm. All the compounds at 5 mg/L had very high bactericidal activity against some of test pathogens even at contact period 1-2 h. 4-(ß-D-Glucopyranosyl-1›4-?-L-rhamnopyranosyloxy)benzyl thiocarboxamide was the most potent, with 99.2 % inhibition toward Shigella dysenteriae and 100 % toward Bacillus cereus, E. coli and Salmonella typhi within 4 h of contact.
- MeSH
- antiinfekční látky farmakologie MeSH
- Bacteria klasifikace účinky léků MeSH
- biotest MeSH
- chromatografie na tenké vrstvě metody MeSH
- glukosidy farmakologie MeSH
- glukosinoláty farmakologie chemie MeSH
- houby klasifikace účinky léků MeSH
- isothiokyanatany farmakologie chemie MeSH
- magnetická rezonanční spektroskopie MeSH
- mikrobiální testy citlivosti metody MeSH
- Moringa oleifera chemie MeSH
- počet mikrobiálních kolonií MeSH
- rostlinné extrakty farmakologie chemie MeSH
- semena rostlinná chemie MeSH
