The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined. Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg-1 thiram. rGSTA3 protein was injected at 20 μg·kg-1 in group B, E and at 50 μg·kg-1 in C, F. Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p < 0.05) expressed in group E and F as compared to group D on day 14 and 23. IL-7, MHCII, TRAF6, TLR3, TLR5, TLR7, and TLR15 determined insignificant in group D compared to group A on day 23. TD occur in an early phase and alleviated in the later period. rGSTA3 protein can prevent apoptosis and repair degraded chondrocytes.
- MeSH
- apoptóza MeSH
- chondrocyty fyziologie MeSH
- erytrocyty fyziologie MeSH
- glutathiontransferasa genetika metabolismus MeSH
- kur domácí imunologie MeSH
- nemoci drůbeže imunologie MeSH
- osteochondrodysplazie imunologie MeSH
- přirozená imunita MeSH
- ptačí proteiny genetika metabolismus MeSH
- rekombinantní proteiny metabolismus MeSH
- signální transdukce genetika MeSH
- thiram metabolismus MeSH
- toll-like receptory metabolismus MeSH
- transkriptom MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- alergie MeSH
- kontaktní dermatitida * etiologie MeSH
- lidé MeSH
- průmyslové fungicidy MeSH
- retrospektivní studie MeSH
- thiram škodlivé účinky MeSH
- zdravotnický personál * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- zprávy MeSH
- MeSH
- alergie MeSH
- kontaktní dermatitida * etiologie MeSH
- lidé MeSH
- průmyslové fungicidy MeSH
- retrospektivní studie MeSH
- thiram škodlivé účinky MeSH
- zdravotnický personál * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- zprávy MeSH
