Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F(2alpha), malondialdehyde and 4-hydroxynonenal. The method consisted of a pre-treatment part a solid-phase extraction, for rapid and effective isolation of biomarkers from body fluids (exhaled breath condensate, plasma and urine) and the detection method LC-ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized by the following parameters: the imprecision was below 14.3%, the mean inaccuracy was determined to be lower than 13.1%. The method was tested on samples obtained from patients diagnosed with asbestosis, pleural hyalinosis or silicosis, i.e. occupational lung diseases caused by fibrogenic dusts, inducing oxidative stress in the respiratory system, and then compared to samples from healthy subjects. The difference in concentration levels of biomarkers between the two groups was perceptible in all the body fluids (the difference observed in an exhaled breath condensate was statistically most significant).
- MeSH
- aldehydy analýza krev moč MeSH
- azbest toxicita MeSH
- biologické markery analýza krev moč MeSH
- dinoprost analogy a deriváty analýza krev moč MeSH
- extrakce na pevné fázi metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- malondialdehyd analýza krev moč MeSH
- oxid křemičitý toxicita MeSH
- oxidační stres MeSH
- plicní nemoci etiologie krev metabolismus moč MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- tělesné tekutiny chemie metabolismus MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- Publikační typ
- hodnotící studie MeSH
- práce podpořená grantem MeSH
