BACKGROUND: A one-step polymerase chain reaction (PCR) method for the simultaneous detection of the major allergens of pecan and Brazil nuts was developed. Primer pairs for the amplification of partial sequences of genes encoding the allergens were designed and tested for their specificity on a range of food components. RESULTS: The targeted amplicon size was 173 bp of Ber e 1 gene of Brazil nuts and 72 bp of vicilin-like seed storage protein gene in pecan nuts. The primer pair detecting the noncoding region of the chloroplast DNA was used as the internal control of amplification. The intrinsic detection limit of the PCR method was 100 pg mL(-1) pecan or Brazil nuts DNA. The practical detection limit was 0.1% w/w (1 g kg(-1)). The method was applied for the investigation of 63 samples with the declaration of pecans, Brazil nuts, other different nut species or nuts generally. In 15 food samples pecans and Brazil nuts allergens were identified in the conformity with the food declaration. CONCLUSION: The presented multiplex PCR method is specific enough and can be used as a fast approach for the detection of major allergens of pecan or Brazil nuts in food.
- MeSH
- 2S albuminy rostlinné škodlivé účinky analýza genetika MeSH
- alergeny analýza genetika MeSH
- alergie na arašídy prevence a kontrola MeSH
- alergie na ořechy prevence a kontrola MeSH
- analýza potravin metody MeSH
- antigeny rostlinné škodlivé účinky analýza genetika MeSH
- Bertholletia škodlivé účinky chemie MeSH
- Carya škodlivé účinky chemie MeSH
- DNA rostlinná metabolismus MeSH
- kontrola potravin metody MeSH
- limita detekce MeSH
- multiplexová polymerázová řetězová reakce MeSH
- ořechy škodlivé účinky chemie MeSH
- zásobní proteiny semen škodlivé účinky analýza genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
