This is the first study carried out to describe the role of fetal microchimerism (FM) in the pathogenesis of uterine cancer. The prevalence and concentration of male fetal microchimeric cells (FMCs) were examined in endometrial tissues in relation to subtypes of uterine cancer, and the histological grade and stage of the tumor. FM occurrence was analyzed in relation to risk factors, including hypertension, obesity, type 2 diabetes, dyslipidemia, age at cancer diagnosis, and patient pregnancy history. The prevalence and concentration of FMCs were examined in endometrial tissues using real-time polymerase chain reaction, SRY and β-globin sequences as markers for male fetal FMCs and total DNA. The studied group involved 47 type 1 endometrial cancers, 28 type 2 endometrial cancers, and 41 benign uterine diseases. While the prevalence of FM was decreased only in type 1 endometrial cancer, compared with benign uterine disorders (38.3% vs.70.7%; odds ratio [OR]=0.257, 95% confidence interval [CI]: 0.105 to 0.628, p=0.003), FMC concentrations did not differ within examined groups. The lower FM prevalence was detected in low-grade (grade 1 and grade 2) endometrioid cancer (38.3% vs. 70.7%, OR=0.256, 95% CI: 0.105 to 0.627, p=0.003) and in FIGO 1 tumors (40.7% vs. 70.7%, OR=0.285, 95% CI: 0.120 to 0.675, p=0.004). No correlation between FM prevalence or FMC concentrations and risk factors was demonstrated. A lower prevalence of male FM seemed to be associated with better prognoses in uterine cancer based on tumor subtype, histological grade, and stage of the tumor.
- MeSH
- časná diagnóza MeSH
- chimérismus statistika a číselné údaje MeSH
- dospělí MeSH
- endometrium cytologie patologie MeSH
- fetální kmenové buňky MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- maternofetální výměna látek genetika MeSH
- nádory dělohy diagnóza genetika patologie MeSH
- nemoci dělohy diagnóza patologie MeSH
- prognóza MeSH
- rizikové faktory MeSH
- senioři MeSH
- staging nádorů MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Evaluation of free plasma DNA has been suggested as one of the option to detect organ rejection in transplanted patients. However, the presence of chimeric cells in organs could make this approach complicated. To study the presence of Y-chromosome chimeric cells, we examined biopsy samples of 40 thoracic aortas of female donors and recipients. Using nested polymerase chain reaction, the presence of Y-chromosome-specific DNA sequences was detected in 19 (47.5%) analyzed tissue samples. Thus, free DNA originating from more than two genomes could be present in plasma in such a high proportion of cases. This phenomenon makes the use of plasma free DNA for the detection of organ rejection difficult and impractical.
- MeSH
- aorta thoracica MeSH
- chimérismus statistika a číselné údaje MeSH
- DNA primery MeSH
- DNA genetika izolace a purifikace krev MeSH
- lidé MeSH
- lidský chromozom Y MeSH
- polymerázová řetězová reakce MeSH
- transplantační chiméra fyziologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
