Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

• MeSH
• Dextrans chemistry   ultrastructure   MeSH
• Humans MeSH
• Magnetite Nanoparticles chemistry   ultrastructure   MeSH
• Molecular Imaging methods   MeSH
• Antibodies, Monoclonal chemistry   immunology   MeSH
• Biomarkers, Tumor analysis   MeSH
• Cell Line, Tumor MeSH
• Prostatic Neoplasms chemistry   diagnosis   immunology   MeSH
• Nanocapsules chemistry   ultrastructure   MeSH
• Reproducibility of Results MeSH
• Fluorescence Resonance Energy Transfer methods   MeSH
• Sarcosine analysis   immunology   MeSH
• Sensitivity and Specificity MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH