BACKGROUND: Bacterial resistance to antibiotics is a constantly growing challenge. Photodynamic therapy (PDT) offers a new approach to the treatment of bacterial and viral diseases. The aim of this study was to compare the efficacy of photosensitizers used in PDT applied to cell lines and bacterial strains. METHODS: We tested the cytotoxicity and phototoxicity of 3 photosensitizers: TPPS4, ZnTPPS4 and TMPyP applied to the NIH3T3 cell line using two established methods for measuring ROS production and, MTT viability assay. Bacterial viability was determined spectrophotometrically over 24 h following PDT. RESULTS: The most efficient photosensitiser was TMPyP as it reduced the viability of the NIH3T3 cell line by more than 85%. In general, the photosensitisers were more phototoxic to the two Gram-positive bacterial strains, Enterococcus faecalis and Staphylococcus aureus. The viability of E. faecalis was reduced to 78 % by a dose radiation 0.5 J/cm(2) and concentration of TMPyP 1.562 μmol/L. The viability of bacterium S. aureus was reduced to 23 % when exposed to a radiation dose 0.5 J/cm(2) and 100 μmol/L concentration of ZnTPPS4. The highest viability decrease (15 %) for Pseudomonas aeruginosa was caused by 0.5 J/cm(2) radiation dose and 50 μmol/L TMPyP concentration. Escherichia coli proved to be PDT resistant as the bacterial viability was higher than 90%. CONCLUSIONS: The goal of the present study was to test the efficiency of photosensitizers on the NIH 3T3 cell line and bacterial cells. Subsequently we would like to study effectiveness of photosensitizers bound to carriers (for example cyclodextrins) on other cell line and bacterial strain.
- MeSH
- buňky NIH 3T3 účinky léků účinky záření MeSH
- Enterococcus faecalis účinky léků účinky záření MeSH
- Escherichia coli účinky léků účinky záření MeSH
- fotochemoterapie * MeSH
- fotosenzibilizující látky farmakologie MeSH
- metaloporfyriny farmakologie MeSH
- mikrobiální viabilita účinky léků účinky záření MeSH
- myši MeSH
- porfyriny farmakologie MeSH
- Pseudomonas aeruginosa účinky léků účinky záření MeSH
- reaktivní formy kyslíku analýza MeSH
- Staphylococcus aureus účinky léků účinky záření MeSH
- viabilita buněk účinky léků účinky záření MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The effect of treatment with inducing agents, such as mitomycin C, hydrogen peroxide and UV irradiation on the production of two inhibitors by different mutants from Enterococcus faecalis S-48 was studied. With hydrogen peroxide and UV light no increase in either the absolute or the relative amount of antagonistic substances was observed. With mitomycin C, a significant increase in the individual cell capacity for inhibitor production was detected.
- MeSH
- antibakteriální látky * MeSH
- bakteriální proteiny * MeSH
- bakteriociny biosyntéza MeSH
- biosyntéza peptidů MeSH
- chemická stimulace MeSH
- Enterococcus faecalis účinky léků metabolismus účinky záření MeSH
- mitomycin MeSH
- mitomyciny farmakologie MeSH
- peptidy genetika MeSH
- peroxid vodíku farmakologie MeSH
- regulace genové exprese u bakterií účinky léků účinky záření MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
