Fytochemická analýza methanolického extraktu Helichrysum petiolare Hilliard & B. L. (Asteraceae) prokázala přítomnost sekundárních metabolitů patřících mezi fenylpropanoidy a flavonoidy. Pomocí preparativní HPLC se podařilo vyizolovat pět obsahových látek: kumarin skopolin (1), kyselinu 3-chlorogenovou (2), glukosidicky vázaný derivát kyseliny kávové (3), kyselinu dikafeoylchinovou (5) a flavonoid isokvercitrin (4). V druhu H. petiolare byly tyto sloučeniny identifikovány poprvé. Při testování antibakteriální aktivity bylo zjištěno, že pouze kyselina dikafeoylchinová vykazuje mírnou antibakteriální aktivitu proti Escherichia coli CCM 7929 v koncentraci 512 μg ∙ ml–1.
The phytochemical analysis of a methanolic extract from Helichrysum petiolare Hilliard & B. L. (Asteraceae) confirmed the content of phenylpropanoids and flavonoids. Five secondary metabolites were isolated using preparative HPLC, namely coumarin scopolin (1), 3-chlorogenic acid (2), caffeic acid-hexose derivative (3), dicaffeoylquinic acid (5), and the flavonoid isoquercitrin (4). These compounds were identified from this species for the first time. Only dicaffeoylquinic acid was able to inhibit Escherichia coli CCM 7929 at the concertation of 512 μg ∙ mL–1 in a screening of antibacterial activity.
- MeSH
- fytoterapie MeSH
- Helichrysum * chemie MeSH
- léčivé rostliny MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
Helichrysum faradifani (Asteraceae) is a perennial shrub growing in rocky and sandy places of Madagascar. The plant is used in the Malagasy traditional medicine as a wound-healing agent, disinfectant and for the treatment of syphilis, diarrhea, cough and headache. In the present work, we analysed the chemical composition of the essential oil distilled from the aerial parts of H. faradifani by GC-MS and evaluated its insecticidal activity against 2nd, 3rd and 4th instar larvae of the lymphatic filariasis vector Culex quinquefasciatus by acute toxicity assays. The most sensitive were 2nd instar (LC50 = 85.7 μL L-1) larvae. For the 3rd and 4th instar larvae, the estimated LC50 were 156.8 and 134.1 μL L-1, respectively. Monoterpene hydrocarbons (51.6%) were the major fraction of the essential oil, with the bicyclic α-fenchene (35.6%) as the predominant component. Sesquiterpene hydrocarbons (34.0%) were the second major group characterising the oil, with γ-curcumene (17.7%) as the most abundant component.
- MeSH
- Culex účinky léků MeSH
- Helichrysum chemie MeSH
- insekticidy chemie farmakologie MeSH
- komáří přenašeči účinky léků MeSH
- larva účinky léků MeSH
- monoterpeny analýza chemie MeSH
- nadzemní části rostlin chemie MeSH
- oleje prchavé chemie farmakologie MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- rostlinné extrakty chemie MeSH
- seskviterpeny analýza chemie MeSH
- testy akutní toxicity MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Madagaskar MeSH
Helichrysum italicum (Roth) G. Don is a flowering plant of the family Asteraceae. It is rich in oil that is used for different medicinal purposes and in fragrance industry. Volatile profile of four populations of H. italicum, collected from natural habitat in Dalmatia (Croatia), was analysed by capillary GC-MS. Sample from BraČ Island had α-trans-bergamotene (10.2%) and β-acoradiene (10.1%) as the majors, whereas sample collected on Biokovo Mt. was rich in neryl acetate (8.1%). β-Acoradiene was also the main constituent of sample collected near Tijarica, whereas rosifoliol (8.5%) was the most abundant constituent in sample collected near Makarska. Presented results show the influence of environmental conditions on chemical differentiation of the volatiles of H. italicum from Croatia.
- MeSH
- Helichrysum chemie MeSH
- můstkové bicyklické sloučeniny analýza MeSH
- nadzemní části rostlin chemie MeSH
- oleje prchavé analýza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- seskviterpeny analýza MeSH
- životní prostředí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Chorvatsko MeSH
The effect of combinations of the crude acetone and aqueous extracts of Helichrysum pedunculatum leaves and eight antibiotics was determined by means of checkerboard and time-kill methods. In the checkerboard method, synergy of 45.8% was observed, being independent of Gram reaction, with combinations in the aqueous extract yielding largely (18.8%) antagonistic interactions. The time-kill assay detected synergy (45.8%) that was also independent of Gram reaction with a potentiation of more than 3 orders of the bactericidal activity of the test antibiotics. The crude leaf extracts of H. pedunculatum could thus be considered to be potential source of a broad-spectrum antibiotic-resistance-modifying compounds.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria účinky léků MeSH
- financování organizované MeSH
- Helichrysum chemie MeSH
- infekce v ráně farmakoterapie mikrobiologie MeSH
- kombinovaná farmakoterapie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- rostlinné extrakty farmakologie MeSH
- synergismus léků MeSH
- Check Tag
- lidé MeSH
