There is increasing pressure on meat producers worldwide due to the need for higher yields and improved meat quality. This is why anabolic androgenic steroids (AAS) have been widely used in most countries, due to their ability to accelerate animal muscle growth. However, out of concern for their side effects, EU states have banned their use and implemented control mechanisms. But they are reaching their limits, and therefore, it is necessary to look for new ways and investigate the mechanism of action of AAS on muscle tissue. This study replicated the administration of banned AAS (testosterone, nandrolone and their combination) and observed their effect on pig muscle. The pig model was purposely chosen for the study, as no such research has been carried out on this species. At the same time, pork is one of the most consumed meats in Europe. It focused on histological changes in muscle structure, specifically the size of muscle fibres and the number of satellite cells per muscle fibre. Furthermore, ultrastructural changes in muscle fibres, the diameter of myofibrils, the number of myofibrils per area, the distance between myofibrils and the size of sarcomeres were examined. The results using the techniques of histology, fluorescent labelling and transmission electron microscopy showed that, after the application of AAS, there is an increase in the diameter of muscle fibres, an increase in the diameter of myofibrils, a decrease in the number of myofibrils per surface area and, in the case of testosterone, an increase in the distance between myofibrils and an increase in the length of sarcomeres. There was also a significant increase in the number of satellite cells per muscle fibre. The detected statistically significant differences between control and experimental groups provide evidence that selected histological parameters could be additional mechanisms for detecting the presence of AAS in pork meat in the future.
- MeSH
- anabolika * farmakologie MeSH
- kosterní svalová vlákna * účinky léků ultrastruktura MeSH
- kosterní svaly účinky léků anatomie a histologie ultrastruktura MeSH
- myofibrily * účinky léků ultrastruktura MeSH
- nandrolon * farmakologie MeSH
- prasata anatomie a histologie MeSH
- sarkomery účinky léků ultrastruktura MeSH
- satelitní buňky kosterního svalu účinky léků ultrastruktura MeSH
- testosteron * farmakologie MeSH
- transmisní elektronová mikroskopie veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
This study was designed to evaluate the effect of chronic administration of quinine on the myocardium of Swiss albino mice. 20 or 30mg quinine/kg b.w. were administered for 30 consecutive days, while the control mice received equal amounts of normal saline. The mice were sacrificed 24 hours after the last administration. Some of the hearts were excised and processed for light microscopic study while some were assayed for malondialdehyde (MDA) and catalase activities. The histological findings indicated that the treated sections of the heart were at variance with the control. The myocardium in the control section presents well organized myofibrils with long cylindrical mononucleated cells in contrast to a dose dependent distorted arrangement of myofibrils and scanty cellular components observed in the treated sections. There was a statistically significant decrement in the weight difference and relative percentage weight of the heart in a dose dependent manner just as the activities of MDA and catalase significantly increased in the treated groups.These findings indicate that chronic administration of quinine may have some deleterious effects on the heart of mice and by extension may affect its function.
- MeSH
- chinin aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- experimenty na zvířatech statistika a číselné údaje MeSH
- histologické techniky metody přístrojové vybavení využití MeSH
- malárie farmakoterapie MeSH
- mikroskopie metody přístrojové vybavení využití MeSH
- muži MeSH
- myofibrily účinky léků MeSH
- myokard chemie metabolismus patologie MeSH
- statistika jako téma metody MeSH
- toxické účinky škodlivé účinky MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
