The disconnect between preclinical and clinical results underscores the imperative for establishing good animal models, then gleaning all available data on efficacy, safety, and potential toxicities associated with a device or drug. Mini pigs are a commonly used animal model for testing orthopedic and dental devices because their skeletons are large enough to accommodate human-sized implants. The challenge comes with the analyses of their hard tissues: current methods are time-consuming, destructive, and largely limited to histological observations made from the analysis of very few tissue sections. We developed and employed cryo-based methods that preserved the microarchitecture and the cellular/molecular integrity of mini pig hard tissues, then demonstrated that the results of these histological, histochemical, immunohistochemical, and dynamic histomorphometric analyses e.g., mineral apposition rates were comparable with similar data from preclinical rodent models. Thus, the ability to assess static and dynamic bone states increases the translational value of mini pig and other large animal model studies. In sum, this method represents logical means to minimize the number of animals in a study while simultaneously maximizing the amount of information collected from each specimen.
- MeSH
- fyziologická kalcifikace MeSH
- kryoprezervace metody MeSH
- kryoultramikrotomie metody MeSH
- lebka cytologie MeSH
- miniaturní prasata MeSH
- odběr biologického vzorku metody MeSH
- polyethylenglykoly MeSH
- prasata MeSH
- remodelace kosti MeSH
- sacharosa MeSH
- sodná sůl karboxymethylcelulosy MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Sulphur (?34S), carbon (?13C) and nitrogen (?15N) isotope data are presented for 120 humans and 33 animals from eight sites dating from the Neolithic to the Iron Age (Benzingerode, Westerhausen, Derenburg, Halberstadt, Karsdorf, Kölsa, Grebehna and Großstorkwitz) from central Germany. Sulphur isotope ratios from skeletal collagen reflect the dietary sulphur isotopic composition and therefore differ between environments and regions. Here it is shown that the majority of the analyzed individuals are consistent with a regional local origin; only three may be of non-local origin, namely one each from Benzingerode, Westerhausen and Derenburg. At Großstorkwitz the ?34S values correlate with the ?15N values and suggest the input of freshwater proteins. At the other sites the ?34S values, along with the ?13C and ?15N values, reflect a terrestrial based diet. No significant difference in ?34S values could be observed through time. Sulphur isotope signatures were not significantly different in animals with different ?15N values and animals are assumed to have lived locally. However, there may not be much regional variability in ?34S in this area, and the distribution of the archaeological ?34S values displays little variation across the investigated landscape.
- Klíčová slova
- Neolit, Doba železná, Bell Beaker, Migrace,
- MeSH
- archeologie * metody MeSH
- dějiny starověku MeSH
- hodnotící studie jako téma MeSH
- izotopy dusíku MeSH
- izotopy síry analýza MeSH
- kolagen MeSH
- kosti a kostní tkáň MeSH
- lebka anatomie a histologie cytologie MeSH
- lidé MeSH
- migrace lidstva * dějiny MeSH
- stravovací zvyklosti * MeSH
- uhlík analýza MeSH
- vývoj člověka MeSH
- zvířata MeSH
- Check Tag
- dějiny starověku MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- historické články MeSH
- Geografické názvy
- Německo MeSH
