In this study a completely stirred tank reactor was used to study the effect of sulfide to nitrate (S/N) ratio on sulfide removal while nitrate was used as electron acceptor. Several S/N ratios were studied for this purpose ranging from 0.3 to 2.4 mol/mol. The complete sulfide removal was achieved when S/N ratio 0.85 mol/mol was used with the autotrophic denitrification efficiency up to 80 %. No nitrite accumulation was observed, and the main product of sulfide oxidation was sulfate. Dissimilatory nitrogen reduction to ammonia occurred and subsequently, elemental sulfur accumulated while S/N ratio was higher than 1.3 mol/mol. The specific autotrophic denitrification rates under S/N ratios 0.8 and 1.2 were 5 and 26 mg g(-1) h(-1) (N-NO3 (-), VSS), respectively. Thiobacillus denitrificans and Thiomicrospira denitrificans were detected in the reactor by fluorescent in situ hybridization, but their overall representation was not more than 5 % of the entire microbial populations.
- MeSH
- amoniak metabolismus MeSH
- bioreaktory mikrobiologie MeSH
- denitrifikace * MeSH
- dusičnany metabolismus MeSH
- dusík analýza MeSH
- Epsilonproteobacteria genetika izolace a purifikace metabolismus MeSH
- hybridizace in situ fluorescenční MeSH
- oxidace-redukce MeSH
- síra analýza MeSH
- sírany metabolismus MeSH
- sulfidy metabolismus MeSH
- Thiobacillus genetika izolace a purifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
