Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the "Rous-like" virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the "HIV-like" virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the "HIV-like", which retained fractional infectivity.
- MeSH
- AIDS opičí virologie MeSH
- Cercopithecus aethiops MeSH
- COS buňky MeSH
- financování organizované MeSH
- genové produkty gag genetika MeSH
- genové produkty pol genetika MeSH
- lidé MeSH
- Masonův-Pfizerův opičí virus genetika patogenita MeSH
- messenger RNA genetika MeSH
- posunová mutace MeSH
- proteosyntéza MeSH
- RNA virová genetika MeSH
- transfekce MeSH
- virion genetika patogenita MeSH
- virové proteiny genetika MeSH
- virus Rousova sarkomu genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
