High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.
- MeSH
- akrozom účinky léků MeSH
- antioxidancia farmakologie MeSH
- buněčná membrána účinky léků MeSH
- cystein farmakologie MeSH
- glutathion farmakologie MeSH
- katalasa farmakologie MeSH
- kryoprezervace metody veterinární MeSH
- kryoprotektivní látky farmakologie MeSH
- kyselina askorbová farmakologie MeSH
- motilita spermií účinky léků MeSH
- reaktivní formy kyslíku analýza MeSH
- ryby * MeSH
- spermie účinky léků MeSH
- uchování spermatu metody veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Nitric oxide (NO) is a major gasotransmitter involved in several physiological processes of male reproduction. There is, nevertheless, little information concerning the role of NO during semen storage. The aim of this study was to evaluate the effect of NO on boar semen stored at 17oC for 72 h. For this purporse, sperm samples were treated with 0.625, 1.25, 2.5, 5, and 10 mM aminoguanidine (AG) or Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), a selective and non-selective NO synthase (NOS) inhibitor, respectively. Moreover, sodium nitroprusside (SNP), a NO donor, was used at the dose of 18.75, 37.5, 75, and 150 μM. Sperm motility, membrane integrity, and acrosomal status were evaluated at 0, 4, 24, 48, and 72 h of semen storage. A significant increase of the amplitude of lateral sperm head displacement (ALH), and both curvilinear and straight-line velocity (VCL and VSL, respectively) was observed at 72 h of semen storage in samples treated with 0.625 mM AG, probably because of the antioxidant properties of this NOS inhibitor. Contrarily, 0.625 mM L-NAME showed no effect on boar sperm parameters during the entire period of semen storage. Moreover, AG and L-NAME at 10 mM negatively affected sperm kinetics and acrosome integrity, which may provide further support to the notion that low NO levels are necessary for a normal sperm function. The concentrations of SNP used in this study had mostly no or negative effects on boar sperm parameters during semen storage. In conclusion, the results from this study increase the understanding of the role of NO on boar sperm physiology.
- MeSH
- akrozom účinky léků MeSH
- buněčná membrána účinky léků MeSH
- časové faktory MeSH
- guanidiny farmakologie MeSH
- motilita spermií účinky léků MeSH
- nitroprusid farmakologie MeSH
- oxid dusnatý aplikace a dávkování farmakologie MeSH
- prasata * MeSH
- spermie účinky léků MeSH
- synthasa oxidu dusnatého, typ II antagonisté a inhibitory metabolismus MeSH
- uchování spermatu veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- akrozom fyziologie účinky léků MeSH
- diethylstilbestrol aplikace a dávkování škodlivé účinky MeSH
- endokrinní disruptory izolace a purifikace metabolismus škodlivé účinky MeSH
- fenoly aplikace a dávkování škodlivé účinky MeSH
- fertilita genetika imunologie účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- financování organizované MeSH
- fytoestrogeny aplikace a dávkování škodlivé účinky MeSH
- myši genetika MeSH
- savci MeSH
- tělesná hmotnost fyziologie účinky léků MeSH
- velikost orgánu fyziologie účinky léků MeSH
- Check Tag
- myši genetika MeSH
