The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices-optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe-act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent-compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers.

• MeSH
• buněčné linie MeSH
• DNA chemie   ultrastruktura   MeSH
• exprese genu MeSH
• fluorescenční protilátková technika MeSH
• genetické vektory * chemie   ultrastruktura   MeSH
• kationty chemie   MeSH
• kovové nanočástice chemie   ultrastruktura   MeSH
• lidé MeSH
• mikroskopie atomárních sil metody   MeSH
• molekulární struktura MeSH
• průtoková cytometrie MeSH
• reportérové geny MeSH
• technika přenosu genů * MeSH
• transfekce MeSH
• viabilita buněk MeSH
• železnaté sloučeniny chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH