We investigated the effect of a Multiwave Locked System laser (with a simultaneous 808 nm continuous emission and 905 nm pulse emission) on the spinal cord after spinal cord injury (SCI) in rats. The functional recovery was measured by locomotor tests (BBB, Beam walking, MotoRater) and a sensitivity test (Plantar test). The locomotor tests showed a significant improvement of the locomotor functions of the rats after laser treatment from the first week following lesioning, compared to the controls. The laser treatment significantly diminished thermal hyperalgesia after SCI as measured by the Plantar test. The atrophy of the soleus muscle was reduced in the laser treated rats. The histopathological investigation showed a positive effect of the laser therapy on white and gray matter sparing. Our data suggests an upregulation of M2 macrophages in laser treated animals by the increasing number of double labeled CD68+/CD206+ cells in the cranial and central parts of the lesion, compared to the control animals. A shift in microglial/macrophage polarization was confirmed by gene expression analysis by significant mRNA downregulation of Cd86 (marker of inflammatory M1), and non-significant upregulation of Arg1 (marker of M2). These results demonstrated that the combination of 808 nm and 905 nm wavelength light is a promising non-invasive therapy for improving functional recovery and tissue sparing after SCI.

• MeSH
• antigeny CD86 genetika   metabolismus   MeSH
• antigeny diferenciační myelomonocytární genetika   metabolismus   MeSH
• CD antigeny genetika   metabolismus   MeSH
• krysa rodu rattus MeSH
• laserová terapie s nízkou intenzitou světla metody   MeSH
• lektiny typu C genetika   metabolismus   MeSH
• lektiny vázající mannosu genetika   metabolismus   MeSH
• lokomoce MeSH
• mícha metabolismus   patologie   MeSH
• poranění míchy terapie   MeSH
• potkani Wistar MeSH
• receptory buněčného povrchu genetika   metabolismus   MeSH
• regenerace míchy MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

One of the industrially important qualities of yeast is their ability to provide the cell-cell and cell-support interactions. This feature of yeast is responsible for technologically significant phenomena such as flocculation (brewing) and yeast biofilm formation (immobilization to supports), whereas these phenomena are time, environment, and strain dependent. Therefore, the goal of this work was to verify the possibility to predict and subsequently select yeast strains capable to colonize solid supports by using physicochemical adhesion models. Three different industrial yeast strains (Saccharomyces cerevisiae) were tested for their adhesion onto spent grain particles in the continuous gas-lift reactor. The cell adhesion energies were calculated, based on physicochemical characteristics of surfaces involved, according to three adhesion models (DLVO theory, thermodynamic approach, and extended DLVO theory). The role of physicochemical surface properties in the cell-cell and cell-support interactions was evaluated by comparing the computed predictions with experimental results. The best agreement between forecast and observation of the yeast adhesion to spent grains was achieved with the extended DLVO (XDLVO) theory, the most complex adhesion model applied in this study. Despite its relative comprehensiveness, the XDLVO theory does not take into account specific biochemical interactions. Consequently, additional understanding of the yeast adhesion mechanism was obtained by means of quantifying the expression of selected FLO genes. The presented approach provides tools to select the appropriately adhesive yeast strains and match them with solid supports of convenient surface properties in order to design immobilized biocatalysts exploitable in biotechnological processes.

• MeSH
• biotechnologie MeSH
• buněčná adheze MeSH
• chemie fyzikální MeSH
• flokulace MeSH
• imobilizované proteiny chemie   MeSH
• lektiny vázající mannosu klasifikace   genetika   metabolismus   MeSH
• molekulární sekvence - údaje MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• povrchové vlastnosti MeSH
• Saccharomyces cerevisiae - proteiny klasifikace   genetika   metabolismus   MeSH
• Saccharomyces cerevisiae genetika   metabolismus   MeSH
• sekvence nukleotidů MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH