OBJECTIVE: A role of autoreactive T cells for type 1 diabetes pathogenesis is considered crucial. In our pilot study we addressed if autoreactive mononuclear cells are present also in peripheral blood of patients with other specific forms of diabetes as cystic fibrosis related diabetes (CFRD). METHODS: Cellular immune responses to a known beta-cell autoantigen (GAD65 and GAD65 derived peptides) were analysed by ELISPOT (IFN-gamma) and by protein microarray analysis in four patients suffering from CFRD, in four cystic fibrosis (CF) patients without diabetes, in eight type 1 diabetes patients (without CF) and in four healthy controls. RESULTS: Response to the autoantigen GAD65 (protein and peptides) was observed in 7/8 patients suffering from CF and in all type 1 diabetes patients. Post-stimulation production of Th1 cytokines (IFN-gamma, TNF-beta) was observed in 2/4 CFRD, 1/4 CF patients and in 7/8 type 1 diabetes patients. All these patients carry prodiabetogenic HLA-DQ genotype. Th2- and Th3 type of cytokine pattern was observed in 2/4 CF patients. Production of IL-8 was observed in the third CFRD as well as in the third CF patient and in 1/8 type 1 diabetes patient and borderline production of this chemokine was also observed in 2/4 healthy controls. No reaction was observed in the other 2/4 healthy controls and in the fourth CFRD patient who carried a strongly protective genotype and did not produce autoantibodies. The most potent peptide of GAD65 was amino acids 509-528. CONCLUSIONS: We consider our observations as a sign of a reaction directed against the self-antigen GAD65 that are closely connected to type 1 diabetes. In CF patients who do not develop diabetes autoreactive mechanisms are very probably efficiently suppressed by immune self-tolerance mechanisms. CFRD patients are a heterogenic group. To disclose those who may display features of autoimmune diabetes could have an impact for their therapy and prognosis.

• MeSH
• autoprotilátky imunologie   krev   MeSH
• čipová analýza proteinů MeSH
• cystická fibróza imunologie   komplikace   MeSH
• diabetes mellitus etiologie   imunologie   MeSH
• dítě MeSH
• dospělí MeSH
• financování organizované MeSH
• glutamát dekarboxyláza imunologie   MeSH
• HLA-DQ antigeny imunologie   MeSH
• interferon gama krev   MeSH
• interleukin-8 krev   MeSH
• izoenzymy MeSH
• leukocyty mononukleární imunologie   MeSH
• lidé MeSH
• lymfotoxin-alfa krev   MeSH
• mladiství MeSH
• pilotní projekty MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

• MeSH
• HLA antigeny krev   MeSH
• lidé MeSH
• lymfotoxin-alfa krev   MeSH
• poporodní období MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH