Siderophores represent important microbial virulence factors and infection biomarkers. Their monitoring in fermentation broths, bodily fluids, and tissues should be reproducible. Similar isolation, characterization, and quantitation studies can often have conflicting results, and without proper documentation of sample collection, data processing, and analysis methods, it is difficult to reexamine the data and reconcile these differences. In this Springer Nature Protocol, we present the procedure optimized for ferricrocin/triacetylfusarinine C extraction from biological material as well as for tissue fixation and cryosectioning for optical microscopy and for both elemental and molecular mass spectrometry imaging. Special attention is paid to siderophore data mining from conventional and product ion mass spectra, liquid chromatography, and mass spectrometry imaging datasets, performed here by our free software called CycloBranch.
- MeSH
- Aspergillus fumigatus metabolismus MeSH
- biologické markery analýza MeSH
- chromatografie kapalinová metody MeSH
- data mining metody MeSH
- datové soubory jako téma MeSH
- ferrichrom analogy a deriváty izolace a purifikace metabolismus MeSH
- fixace tkání metody MeSH
- hmotnostní spektrometrie metody MeSH
- invazivní plicní aspergilóza diagnóza mikrobiologie MeSH
- kryoultramikrotomie metody MeSH
- krysa rodu rattus MeSH
- kyseliny hydroxamové izolace a purifikace metabolismus MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- siderofory izolace a purifikace metabolismus MeSH
- software MeSH
- železité sloučeniny izolace a purifikace metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
