Broad changes in human innate and adaptive immunity are associated with advanced age. The age-related alteration of gene expression was reported for both T and B lymphocytes. We analysed the genome-wide expression profiles (n=20) of naive and whole B cell populations from young and early aged healthy donors under 60 years. We revealed large homogeneity of all analysed genome-wide expression profiles but did not identified any significant gene deregulation between young (30-45 years) and early aged healthy donors (50-60 years). We argue that B cells avoid the aging program on molecular level until 60 years of age. Our results demonstrate the potential of hematopoietic stem cells to generate uncompromised lymphocytes in early elderly. These are very encouraging findings for the general health and the immunity maintenance would not need any intervention to naive B cells. Rather, a suitable immune stimulation in healthy body environment warrants further research into aging of older elderly.

• MeSH
• Adaptive Immunity genetics   immunology   MeSH
• B-Lymphocytes immunology   metabolism   MeSH
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Immunity, Innate genetics   immunology   MeSH
• Interleukin-7 Receptor alpha Subunit genetics   immunology   metabolism   MeSH
• Gene Expression Profiling methods   MeSH
• Aging genetics   immunology   MeSH
• Transcriptome genetics   immunology   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

In this study we were interested in identification of new markers of chicken response to Salmonella Enteritidis infection. To reach this aim, gene expression in the spleens of naive chickens and those intravenously infected with S. Enteritidis with or without previous oral vaccination was determined by 454 pyrosequencing of splenic mRNA/cDNA. Forty genes with increased expression at the level of transcription were identified. The most inducible genes encoded avidin (AVD), extracellular fatty acid binding protein (EXFABP), immune responsive gene 1 (IRG1), chemokine ah221 (AH221), trappin-6-like protein (TRAP6) and serum amyloid A (SAA). Using cDNA from sorted splenic B-lymphocytes, macrophages, CD4, CD8 and γδ T-lymphocytes, we found that the above mentioned genes were preferentially expressed in macrophages. AVD, EXFABP, IRG1, AH221, TRAP6 and SAA were induced also in the cecum of chickens orally infected with S. Enteritidis on day 1 of life or day 42 of life. Unusual results were obtained for the immunoglobulin encoding transcripts. Prior to the infection, transcripts coding for the constant parts of IgM, IgY, IgA and Ig light chain were detected in B-lymphocytes. However, after the infection, immunoglobulin encoding transcripts were expressed also by T-lymphocytes and macrophages. Expression of AVD, EXFABP, IRG1, AH221, TRAP6, SAA and all immunoglobulin genes can be therefore used for the characterization of the course of S. Enteritidis infection in chickens.

• MeSH
• B-Lymphocytes immunology   metabolism   MeSH
• Cecum immunology   metabolism   MeSH
• Immunoglobulins genetics   immunology   MeSH
• Chickens genetics   immunology   MeSH
• Macrophages immunology   metabolism   MeSH
• RNA, Messenger biosynthesis   genetics   MeSH
• Poultry Diseases genetics   immunology   microbiology   MeSH
• Organ Specificity MeSH
• Avian Proteins genetics   immunology   MeSH
• Gene Expression Regulation MeSH
• Salmonella enteritidis immunology   pathogenicity   MeSH
• Salmonella Infections, Animal genetics   immunology   microbiology   MeSH
• Sequence Analysis, DNA MeSH
• Spleen immunology   metabolism   MeSH
• T-Lymphocytes immunology   metabolism   MeSH
• Transcriptome genetics   immunology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH