Rostoucí množství sbíraných dat a vývoj nových technologií s sebou přináší změny v přístupu k datům. Mění se i pohled na samotné softwary, jež s daty pracují. Obstojí tradiční přístupy k vyhodnocení dat? Jaké jsou současné trendy v oblasti nástrojů pro zpracování dat? A co bude dál? Trendy v oblasti nástrojů pro analýzu dat Vám ukáže přímo v softwaru konzultant analytické platformy Statistica – Ing. Miloš Uldrich. To vše se zaměřením na analýzu dat v oblasti medicíny.
Increased amount of data collected, development of new technologies as well as need of quick decisions brings changes in the field of data processing. This implies development of analytical platforms, including new features or even different approaches for working in the software. What are the current trends in tools for data processing? What about traditional approaches? And what will be next? Trends in data analysis tools will be shown by software consultant of analytic platform Statistica - Ing. Milos Uldrich. Contribution will be focused on data and problems from medical sector.
Giardia intestinalis is an important single-celled human pathogen. Interestingly, this organism has two equal-sized transcriptionally active nuclei, each considered diploid. By evaluating condensed chromosome numbers and visualizing homologous chromosomes by fluorescent in situ hybridization, we determined that the Giardia cells are constitutively aneuploid. We observed karyotype inter-and intra-population heterogeneity in eight cell lines from two clinical isolates, suggesting constant karyotype evolution during in vitro cultivation. High levels of chromosomal instability and frequent mitotic missegregations observed in four cell lines correlated with a proliferative disadvantage and growth retardation. Other cell lines, although derived from the same clinical isolate, revealed a stable yet aneuploid karyotype. We suggest that both chromatid missegregations and structural rearrangements contribute to shaping the Giardia genome, leading to whole-chromosome aneuploidy, unequal gene distribution, and a genomic divergence of the two nuclei within one cell. Aneuploidy in Giardia is further propagated without p53-mediated cell cycle arrest and might have been a key mechanism in generating the genetic diversity of this human pathogen.
- MeSH
- aneuploidie * MeSH
- buněčné dělení fyziologie MeSH
- buněčné jádro metabolismus MeSH
- chromozomální nestabilita genetika MeSH
- genetická variace genetika MeSH
- genom protozoální genetika MeSH
- Giardia lamblia genetika izolace a purifikace MeSH
- hybridizace in situ fluorescenční MeSH
- karyotyp MeSH
- kontrolní body buněčného cyklu genetika MeSH
- lidé MeSH
- proliferace buněk genetika MeSH
- segregace chromozomů genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
