Trees are carbon dioxide sinks and major producers of terrestrial biomass with distinct seasonal growth patterns. Circadian clocks enable the coordination of physiological and biochemical temporal activities, optimally regulating multiple traits including growth. To dissect the clock's role in growth, we analysed Populus tremula × P. tremuloides trees with impaired clock function due to down-regulation of central clock components. late elongated hypocotyl (lhy-10) trees, in which expression of LHY1 and LHY2 is reduced by RNAi, have a short free-running period and show disrupted temporal regulation of gene expression and reduced growth, producing 30-40% less biomass than wild-type trees. Genes important in growth regulation were expressed with an earlier phase in lhy-10, and CYCLIN D3 expression was misaligned and arrhythmic. Levels of cytokinins were lower in lhy-10 trees, which also showed a change in the time of peak expression of genes associated with cell division and growth. However, auxin levels were not altered in lhy-10 trees, and the size of the lignification zone in the stem showed a relative increase. The reduced growth rate and anatomical features of lhy-10 trees were mainly caused by misregulation of cell division, which may have resulted from impaired clock function.

• MeSH
• biomasa MeSH
• buněčné dělení genetika   MeSH
• cirkadiánní hodiny genetika   MeSH
• cytokininy metabolismus   MeSH
• geneticky modifikované rostliny MeSH
• kambium fyziologie   MeSH
• kyseliny indoloctové metabolismus   MeSH
• lignin metabolismus   MeSH
• metabolom MeSH
• metabolomika MeSH
• mutace genetika   MeSH
• Populus cytologie   genetika   růst a vývoj   MeSH
• regulace genové exprese u rostlin * MeSH
• RNA interference MeSH
• rostlinné proteiny genetika   metabolismus   MeSH
• stromy cytologie   genetika   růst a vývoj   MeSH
• vazba proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH