Phytophthora is arguably one of the most damaging genera of plant pathogens. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for reducing its economic and ecological impact. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened for species-specific peptide patterns. Next, Phytophthora proteins were detected in planta, employing model plants Solanum tuberosum and Hordeum vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection, the comparison between qPCR and protein data highlighted more than 300 protein markers, which correlated positively with the amount of P. infestans DNA. Finally, the analysis of P. palmivora response in barley revealed significant alterations in plant metabolism. These changes included enzymes of cell wall metabolism, ROS production, and proteins involved in trafficking. The observed root-specific attenuation in stress-response mechanisms, including the biosynthesis of jasmonates, ethylene and polyamines, and an accumulation of serotonin, provided the first insight into molecular mechanisms behind this particular biotic interaction.

• MeSH
• chromatografie kapalinová MeSH
• fyziologický stres MeSH
• hmotnostní spektrometrie MeSH
• ječmen (rod) enzymologie   metabolismus   mikrobiologie   MeSH
• listy rostlin metabolismus   mikrobiologie   MeSH
• metabolické sítě a dráhy MeSH
• nemoci rostlin mikrobiologie   MeSH
• peptidy metabolismus   MeSH
• Phytophthora infestans genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce MeSH
• proteom metabolismus   MeSH
• proteomika MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• rostlinné proteiny metabolismus   MeSH
• Solanum tuberosum metabolismus   mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH

The genome sequence of a mitovirus found in an isolate of Diaporthe rudis, one of the causal agents of Phomopsis dieback on grapevines, was determined by two high-throughput sequencing approaches, small RNA and total RNA sequencing. The genome of this mitovirus is 2,455 nt in length and includes a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTx comparison of the full-length genome sequence showed the highest similarity (54.15%) with that of Colletotrichum falcatum mitovirus 1 (CfMV1). Our results reveal a new member of the genus Mitovirus first detected in D. rudis (Fr.) Nitschke, with the proposed name "Diaporthe rudis mitovirus 1" (DrMV1).

• MeSH
• délka genomu MeSH
• exprese genu MeSH
• fylogeneze * MeSH
• genom virový * MeSH
• mykoviry klasifikace   genetika   izolace a purifikace   MeSH
• nemoci rostlin mikrobiologie   MeSH
• otevřené čtecí rámce MeSH
• RNA-dependentní RNA-polymerasa genetika   MeSH
• Saccharomycetales virologie   MeSH
• sekvenování celého genomu MeSH
• virové proteiny genetika   MeSH
• Vitis mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH