The aim of this contribution is to provide an overview of comparison of two types of eye lens dosemeter systems. The comparison was performed at the Department of Intravenous Cardiology using patient and physician phantoms and supplemented by dose simulation using the Monte Carlo method. The tests were performed in several specific geometries and in addition to eye lens dosemeters the value of a personal dosemeter located at the reference point was also monitored. The value of Hp(3) and Hp(10) was monitored. It is clear from the results that film dosemeters achieve more correct results in most cases. It is probably due to a better correction for the angle of exposure. This assumption must be verified by more detailed measurements in laboratory conditions.

• MeSH
• dávka záření MeSH
• intervenční radiologie metody   MeSH
• kardiologie * metody   MeSH
• lidé MeSH
• oční čočka * MeSH
• pracovní expozice * analýza   MeSH
• radiační ochrana * metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

LET spectra can be measured by track-etched detectors. However, these detectors are not able to identify the type of interacting particles. Monte Carlo simulations can provide this missing information. In this work, Monte Carlo simulations based on the EURADOS Work Group 9 experiment consisting of systematic 3D mapping of out-of-field doses and LET spectra in a prototype water phantom were performed. The simulations aimed to identify the types of particles contributing to the out-of-field LET spectra. The total absorbed dose, LET and energy spectra were calculated. The calculated dose distributions and LET spectra were compared with the ones measured by radiophotoluminiscence and track-etched detectors. The out-of-field particles and their LET values were identified. No statistically significant differences between the measured and simulated spectra were revealed in the LET range of 100-2000 keV μm-1.

• MeSH
• celková dávka radioterapie MeSH
• lineární přenos energie MeSH
• metoda Monte Carlo MeSH
• protonová terapie * MeSH
• protony * MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH

Iron-based nanomaterials have high technological impacts on various pro-environmental applications, including wastewater treatment using the co-precipitation method. The purpose of this research was to identify the changes of iron nanomaterial's structure caused by the presence of selenium, a typical water contaminant, which might affect the removal when the iron co-precipitation method is used. Therefore, we have investigated the maturation of co-precipitated nanosized ferric oxyhydroxides under alkaline conditions and their thermal transformation into hematite in the presence of selenite and selenate with high concentrations. Since the association of selenium with precipitates surfaces has been proven to be weak, the mineralogy of the system was affected insignificantly, and the goethite was identified as an only ferric phase in all treatments. However, the morphology and the crystallinity of ferric oxyhydroxides was slightly altered. Selenium affected the structural order of precipitates, especially at the initial phase of co-precipitation. Still, the crystal integrity and homogeneity increased with time almost constantly, regardless of the treatment. The thermal transformation into well crystalized hematite was more pronounced in the presence of selenite, while selenate-treated and selenium-free samples indicated the presence of highly disordered fraction. This highlights that the aftermath of selenium release does not result in destabilization of ferric phases; however, since weak interactions of selenium are dominant at alkaline conditions with goethite's surfaces, it still poses a high risk for the environment. The findings of this study should be applicable in waters affected by mining and metallurgical operations.

• MeSH
• alkálie chemie   MeSH
• chemická precipitace MeSH
• krystalizace MeSH
• kyselina seleničitá chemie   MeSH
• kyselina selenová chemie   MeSH
• minerály chemie   MeSH
• sloučeniny železa chemie   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• spektroskopie Mossbauerova MeSH
• teplota MeSH
• železité sloučeniny chemie   MeSH
• železo chemie   MeSH
• Publikační typ
• časopisecké články MeSH

Ms1 is a sRNA recently found in mycobacteria and several other actinobacterial species. Ms1 interacts with the RNA polymerase (RNAP) core devoid of sigma factors, which differs from 6S RNA that binds to RNAP holoenzymes containing the primary sigma factor. Here we show that Ms1 is the most abundant non-rRNA transcript in stationary phase in Mycobacterium smegmatis. The accumulation of Ms1 stems from its high-level synthesis combined with decreased degradation. We identify the Ms1 promoter, PMs1 , and cis-acting elements important for its activity. Furthermore, we demonstrate that PNPase (an RNase) contributes to the differential accumulation of Ms1 during growth. Then, by comparing the transcriptomes of wt and ΔMs1 strains from stationary phase, we reveal that Ms1 affects the intracellular level of RNAP. The absence of Ms1 results in decreased levels of the mRNAs encoding β and β' subunits of RNAP, which is also reflected at the protein level. Thus, the ΔMs1 strain has a smaller pool of RNAPs available when the transcriptional demand increases. This contributes to the inability of the ΔMs1 strain to rapidly react to environmental changes during outgrowth from stationary phase.

• MeSH
• bakteriální RNA metabolismus   MeSH
• delece genu MeSH
• DNA řízené RNA-polymerasy metabolismus   MeSH
• malá nekódující RNA genetika   metabolismus   MeSH
• Mycobacterium smegmatis enzymologie   genetika   růst a vývoj   metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH