DMAP
Dotaz
Zobrazit nápovědu
- Klíčová slova
- Toxogonin, Toluidinblau, Technecistan-(99MTC) sodný, Legalon Sil, Bleomedac, 4-DMAP,
- MeSH
- nádory diagnóza farmakoterapie terapie MeSH
- otrava farmakoterapie terapie MeSH
- schvalování léčiv MeSH
Macromolecular conjugates of a natural polysaccharide, hyaluronic acid, with diethylenetriaminepentaacetic acid (DTPA)-metal complexes were synthesized and characterized by FTIR, NMR, SEC-MALLS and ICP analysis. Several parameters of the cross-linking reaction as molecular weight of starting HA, temperature, equivalent of DTPA bis-anhydride, concentration of HA, presence of transacylation catalyst DMAP and reaction time were studied. The mechanism for the reaction was suggested and relationship between the molecular weight assigned by SEC-MALLS, reaction parameters and rheological properties of the final cross-linked products were investigated.
Prions are suspected as causative agents of several neuropathogenic diseases, even though the mode of their action is still not clear. A combination of chemical and recombinant syntheses can provide suitable probes for explanation of prions role in pathogenesis of neurodegenerative diseases. However, the prions contain several difficult sequences for synthesis by Fmoc/tBu approach. For that reason, the peptide thioesters as the key building blocks for chemical syntheses of proteins by native chemical ligation were employed. A scan of the mouse prion domain 93-231 was carried out in order to discover availability of derived thioesters as the suitable building blocks for a total chemical synthesis of the prion protein based probes. The synthesis on 2-chlorotritylchloride resin was utilized and after a deprotection of the samples for analysis, the peptide segments were purified and characterized. If the problems were detected during the synthesis, the segment was re-synthesized either using the special pseudoproline dipeptides or by splitting its molecule to two or three smaller segments, which were prepared easier. The protected segments, prepared correctly without any deletion and in sufficient amounts, were coupled either with EtSH after DIC/DMAP activation or with p-Ac-NH-Ph-SH using PyBOP activation to yield corresponding thioesters. In some special cases, the other techniques of thioester formation, like sulfonamide-safety catch and/or trimethylaluminium approach were utilized.
- MeSH
- estery chemická syntéza chemie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- myši MeSH
- peptidové fragmenty chemická syntéza chemie MeSH
- priony chemická syntéza chemie MeSH
- proteolýza MeSH
- sekvence aminokyselin MeSH
- sulfidy chemická syntéza chemie MeSH
- techniky syntézy na pevné fázi MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
... aerosol 302 -- Diazepam 302 -- Digoxin 303 -- Dihydralazin 303 -- Dimeticon 303 -- Dimetinden 304 -- 4-DMAP ...
1.vyd. 345 s.
Interdisciplinární kompendium první pomoci pro lékaře Obsah OCHuKlinická medicína. Diagnostika. Anesteziologie. Urgentní medicína. Resuscitace. Experimentální medicína. Vyšetřovací metody. Výzkumy. Lékařská chemie a biochemie. Enzymy. Nukleární medicína. Chemoterapie. Bolest. Onkologie. Osteoporóza. Imunologie. Revmatologie. Hematologie. Alergologie. Popáleniny.
- Klíčová slova
- akutní medicína,
- Publikační typ
- přehledy MeSH
... aerosol 302 -- Diazepam 302 -- Digoxin 303 -- Dihydralazin 303 -- Dimeticon 303 -- Dimetinden 304 -- 4-DMAP ...
345 s. : obr., tab.
Interdisciplinární kompendium první pomoci pro lékaře Obsah OCHuKlinická medicína. Diagnostika. Anesteziologie. Urgentní medicína. Resuscitace. Experimentální medicína. Vyšetřovací metody. Výzkumy. Lékařská chemie a biochemie. Enzymy. Nukleární medicína. Chemoterapie. Bolest. Onkologie. Osteoporóza. Imunologie. Revmatologie. Hematologie. Alergologie. Popáleniny.
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- anesteziologie a intenzivní lékařství
- lékařství
