Oncology, ISSN 0030-2414 vol. 43, suppl. 1, December 1986
70 s. : tab., grafy ; 28 cm
Host size and distance from an infected plant have been previously found to affect mistletoe occurrence in woody vegetation but the effect of host plant competition on mistletoe infection has not been empirically tested. For an individual tree, increasing competition from neighbouring trees decreases its resource availability, and resource availability is also known to affect the establishment of mistletoes on host trees. Therefore, competition is likely to affect mistletoe infection but evidence for such a mechanism is lacking. Based on this, we hypothesised that the probability of occurrence as well as the abundance of mistletoes on a tree would increase not only with increasing host size and decreasing distance from an infected tree but also with decreasing competition by neighbouring trees. Our hypothesis was tested using generalized linear models (GLMs) with data on Loranthus europaeus Jacq., one of the two most common mistletoes in Europe, on 1015 potential host stems collected in a large fully mapped plot in the Czech Republic. Because many trees were multi-stemmed, we ran the analyses for both individual stems and whole trees. We found that the probability of mistletoe occurrence on individual stems was affected mostly by stem size, whereas competition had the most important effects on the probability of mistletoe occurrence on whole trees as well as on mistletoe abundance. Therefore, we confirmed our hypothesis that competition among trees has a negative effect on mistletoe occurrence.
- MeSH
- Quercus * MeSH
- Ecosystem * MeSH
- Forests * MeSH
- Mistletoe * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
New strategies for the fast, efficient, and environmentally friendly extraction of proteins are required to isolate desired bioactive compounds from a technological point of view. In this study, utilization of the pressurized water extraction (PWE) at low temperature (40 °C) for isolation of mistletoe proteins was investigated. PWE effectiveness, based on protein fingerprints, were compared with those obtained by conventional extractions using 10 mmol L-1 Tris-HCl buffer pH 8.3, 50 mmol L-1 phosphate buffer pH 7, or deionized water. The extracts were precipitated using acetone, trichloroacetic acid (TCA), and 20% (w/v) TCA/acetone and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. PWE was more or equally efficient for isolation of mistletoe proteins than evaluated conventional extraction methods. The proteomic analysis combining mass spectrometry and database searching confirmed the presence of 35 proteins in PWE extracts precipitated by acetone, which was the most compounds identified from all studied extracts. The PWE high extraction power was revealed for multiple viscotoxin isoforms and specific enzymes indispensable for the synthesis of terpenes.
- MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Plant Leaves MeSH
- Proteomics MeSH
- Viscum album * MeSH
- Water MeSH
- Publication type
- Journal Article MeSH
- Keywords
- vlaštovičník větší, Chelidonium majus, jmelí bílé, rosolovka mozkovitá, Tremella mezenterica,
- MeSH
- Chelidonium MeSH
- Phytotherapy MeSH
- Fungi MeSH
- Plants, Medicinal * MeSH
- Humans MeSH
- Mistletoe MeSH
- Viscum album MeSH
- Check Tag
- Humans MeSH
Forschende Komplementärmedizin ; Bd. 3, Spl. 1
1. Aufl. 20 s.
- MeSH
- Viscum album immunology MeSH
- Publication type
- Congress MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- alergologie a imunologie
