Ocular tissue engineering
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Stem cell (SC) therapy represents a promising approach to treat a wide variety of injuries, inherited diseases, or acquired SC deficiencies. One of the major problems associated with SC therapy remains the absence of a suitable matrix for SC growth and transfer. We describe here the growth and metabolic characteristics of mouse limbal stem cells (LSCs) and mesenchymal stem cells (MSCs) growing on 3D nanofiber scaffolds fabricated from polyamide 6/12 (PA6/12). The nanofibers were prepared by the original needleless electrospun Nanospider technology, which enables to create nanofibers of defined diameter, porosity, and a basis weight. Copolymer PA6/12 was selected on the basis of the stability of its nanofibers in aqueous solutions, its biocompatibility, and its superior properties as a matrix for the growth of LSCs, MSCs, and corneal epithelial and endothelial cell lines. The morphology, growth properties, and viability of cells grown on PA6/12 nanofibers were comparable with those grown on plastic. LSCs labeled with the fluorescent dye PKH26 and grown on PA6/12 nanofibers were transferred onto the damaged ocular surface, where their seeding and survival were monitored. Cotransfer of LSCs with MSCs, which have immunosuppressive properties, significantly inhibited local inflammatory reactions and supported the healing process. The results thus show that nanofibers prepared from copolymer PA6/12 represent a convenient scaffold for growth of LSCs and MSCs and transfer to treat SC deficiencies and various ocular surface injuries.
- MeSH
- buněčná diferenciace MeSH
- kaprolaktam analogy a deriváty chemie MeSH
- kmenové buňky cytologie MeSH
- kultivované buňky MeSH
- limbus corneae cytologie MeSH
- mezenchymální kmenové buňky cytologie MeSH
- myši MeSH
- nanovlákna MeSH
- polymery chemie MeSH
- poranění oka terapie MeSH
- proliferace buněk MeSH
- rohovkový epitel cytologie MeSH
- tkáňové inženýrství MeSH
- tkáňové podpůrné struktury MeSH
- transplantace kmenových buněk MeSH
- transplantace mezenchymálních kmenových buněk MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The p75 neurotrophin receptor (p75NTR) can play different roles in cells. This protein can on the one hand act in the regulation of cell growth and survival, while being an apoptosis inducing factor in different contexts. p75NTR regulates cell cycle not only in nerve cells but also in epithelial oral mucosal cells. In the former, neurotrophin-p75NTR signaling affects cell growth and survival. Recent studies showed that p75NTR is expressed in basal cells of oral mucosal epithelium and can be used as one of the markers of epithelial stem/progenitor cells. This role of p75NTR can be utilised in aspects such as tissue engineering and gene therapy. One of the examples of clinical use of cultivated oral mucosal cells is ocular surface reconstruction. p75NTR can be a significant marker of stem cells in studies of epithelial tissues, especially when the cells will exhibit other specific markers, such as CK13, CK14 and PCNA..
- MeSH
- epitel metabolismus MeSH
- lidé MeSH
- neurony MeSH
- nízkoafinní receptor faktorů růstu nervů metabolismus MeSH
- signální transdukce MeSH
- ústní sliznice metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- dopisy MeSH
... INTERACTION MECHANISMS 80 -- 6.1 General 80 -- 6.2 Electrical properties of cells and tissues 81 -- 6.2.1 ... ... Body impedance 86 -- 6.3 Direct interactions - strong fields 88 -- 6.3.1 Interactions with excitable tissues ... ... 107 -- 7.2.3 Isolated cerebral tissue, peripheral nerve tissue, and heart preparations 110 -- 7.2.4 ... ... conclusions: in vitro studies 114 -- 7.3 Animal studies 114 -- 7.3.1 Nervous system 115 -- 7.3.2 Ocular ... ... PROTECTIVE MEASURES 187 -- 11.1 Engineering measures 187 -- 11.2 Administrative controls 190 -- 11.3 ...
Environmental health criteria ; Vol. 137
290 s. : obr., tab., bibliogr.
Retinoblastoma (Rb) is the most common primary malignant intraocular tumor in children which develops from the retinal stem cells. Systemic chemotherapy is the typical therapeutic treatment and though most children survive Rb, they often lose their vision, or the eye needs to be enucleated. Regarding to the pure availability of the target tumor by systemic chemotherapy, the local anticancer drug administration would be advantageous to increase the local drug concentration and minimize adverse side effects of chemotherapy. The present paper describes a new hydrogel implant enabled to deliver therapeutically active doses of low molecular weight hydrophilic antitumor drugs topotecan and vincristine. The hydrogel implant is proposed as bi-layered with an inner hydrophilic layer from 2-hydroxyethyl methacrylate (HEMA) serving as a reservoir of the chemotherapeutic agent and an outer hydrophobic layer from 2-ethoxyethyl methacrylate (EOEMA) acting as a barrier to protect the surrounding vascularized tissue against cytotoxicity of the delivered chemotherapeutics. The experiments with enucleated pig eyes demonstrated the ability of tested drugs to diffuse through sclera and reach the vitreous humor. HEMA-based hydrogels were examined in terms of sorption, release and transport properties, showing the possibility of adjusting the loading capacity and diffusion of the drugs by the degree of crosslinking. The EOEMA-based gels proved to be an inert for drug sorption and diffusion. A chorioallantoic membrane assay demonstrated excellent biocompatibility of unloaded hydrogels, and in vitro experiments confirmed significant cytotoxicity of drug-loaded hydrogels against a Rb cell line; 2 days for those topotecan-loaded and a minimum of 6 days for vincristine-loaded hydrogels. The bi-layered hydrogel implant can be considered promising for local administration of active agents to eye-globe for the treatment of Rb and also other ocular disorders.
- MeSH
- hydrogely chemie MeSH
- kinetika MeSH
- lidé MeSH
- methakryláty chemie MeSH
- nádorové buněčné linie MeSH
- nosiče léků chemie MeSH
- oči účinky léků metabolismus MeSH
- prasata MeSH
- protézy a implantáty MeSH
- retinoblastom metabolismus patologie MeSH
- stabilita léku MeSH
- topotekan chemie metabolismus farmakologie MeSH
- viabilita buněk účinky léků MeSH
- vinkristin chemie metabolismus farmakologie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
... Methanol in blood 21 -- 2.4.3.3 Methanol in urine 24 -- 2.4.3.4 Methanol in miscellaneous biological tissues ... ... hepatocytes -- 7.8.2 Toxic interactions -- 7.8.3 Studies with exhaust emissions from methanol-fiielled engines ... ... -- 7.9 Mechanism of ocular toxicity -- 91 -- 92 -- 93 93 -- 93 -- 94 94 -- 8. ...
Environmental health criteria, ISSN 0250-863X No. 196
XVIII, 180 s. : il. ; 24 cm
