Kazuistika pojednává o 16leté dívce vyšetřované pro bolesti břicha, u které byla prokázaná trombóza pánevních žil vlevo a dolní duté žíly navazující na femoropopliteální trombózu. Její stav komplikoval rozvoj plicní embolie. Pacientka byla léčena nízkomolekulárním heparinem s následnou warfarinizací a lokální trombolýzou se zavedením kaválního filtru.

The case report deals with 16-year-old girl examined for abdominal pain who was diagnosed with thrombosis of the left iliac veins and inferior vena cava connected to femoropopliteal thrombosis. Her condition was complicated by the development of pulmonary embolism. The patient was treated with low-molecular-weight heparin followed by oral anticoagulation vitamin K agonist and local thrombolysis with the insertion of caval filter.

• MeSH
• bolesti břicha etiologie   MeSH
• lidé MeSH
• mladiství MeSH
• plicní embolie diagnóza   terapie   MeSH
• počítačová rentgenová tomografie MeSH
• výsledek terapie MeSH
• žilní tromboembolie diagnóza   terapie   MeSH
• žilní trombóza * diagnóza   terapie   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

• Publikační typ
• abstrakt z konference MeSH

INTRODUCTION: Cytochrome (CYP) epoxygenases (CYP2C and CYP2J) and soluble epoxide hydrolase (sEH) participate in the metabolism of arachidonic acid and may also have a potential role in enterocyte differentiation. The first critical step in the study of intestinal cell differentiation is the determination of a suitable in vitro model, which must be as similar as possible to the conditions of a living organism. It is known that HT-29 and Caco2 cell lines derived from human colorectal carcinomas can differentiate into enterocyte-like cells in appropriate culture conditions. MATERIAL AND METHODS: We tested 4 different approaches of enterocyte-like differentiation and determined the most appropriate culture conditions for each model. Subsequently, the changes in the expression of CYP epoxygenases and sEH in undifferentiated and differentiated cells were measured by In-Cell ELISA. These results were compared with immunohistochemical profiles of expression of CYP epoxygenases and sEH in samples of human embryonic and fetal intestines as well as adult duodenum and colon. RESULTS: Our results show that sodium butyrate (NaBt)-differentiated HT-29 cells and spontaneously differentiated Caco2 cells resemble CYP epoxygenases and sEH profiles, corresponding with different types of intestines. CONCLUSION: Our study revealed that the most suitable models for the study of the role of CYP epoxygenases and sEH expression in differentiation of intestinal epithelium are NaBt-differentiated HT-29 cells and spontaneously differentiated Caco2 cells.

• MeSH
• buněčná diferenciace * MeSH
• buňky HT-29 MeSH
• Caco-2 buňky MeSH
• enterocyty enzymologie   MeSH
• epoxid hydrolasy metabolismus   MeSH
• kyselina arachidonová metabolismus   MeSH
• lidé MeSH
• střeva cytologie   embryologie   MeSH
• střevní sliznice * embryologie   metabolismus   MeSH
• systém (enzymů) cytochromů P-450 metabolismus   MeSH
• techniky in vitro MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH