The thioredoxin system is a significant redox regulator in all organisms. Thioredoxins in bacteria are the major dithiol reductants in the cytosol (or an advanced equivalent to dithiotreitol of cells) thanks to the low redox potentials (Holmgren, 1985). In the genome of the studied model Streptomyces coelicolor A3(2) several genes were revealed which code proteins forming the thioredoxin system. It seems that this gram-positive soil bacteria have a very complex redox system, with a variety of reducing possibilities. In this work cloning, purification and characterization of further thioredoxins (TrxA2 and TrxA3) are described. Both proteins were overexpressed in E. coli cytoplasma as soluble active hexahistidine fusion proteins and isolated as homogenous substances.
Bacillus subtilis, a Gram-positive bacterium commonly found in soil, is an excellent model organism for the study of basic cell processes, such as cell division and cell differentiation, called sporulation. In B. subtilis the essential genetic information is carried on a single circular chromosome, the correct segregation of which is crucial for both vegetative growth and sporulation. The proper completion of life cycle requires each daughter cell to obtain identical genetic information. The consequences of inaccurate chromosome segregation can lead to formation of anucleate cells, cells with two chromosomes, or cells with incomplete chromosomes. Although bacteria miss the classical eukaryotic mitotic apparatus, the chromosome segregation is undeniably an active process tightly connected to other cell processes as DNA replication and compaction. To fully understand the chromosome segregation, it is necessary to study this process in a wider context and to examine the role of different proteins at various cell life cycle stages. The life cycle of B. subtilis is characteristic by its specific cell differentiation process where, two slightly different segregation mechanisms exist, specialized in vegetative growth and in sporulation.
- MeSH
- Bacillus subtilis genetics isolation & purification MeSH
- Chromosomal Proteins, Non-Histone genetics isolation & purification MeSH
- Financing, Organized utilization MeSH
- Gram-Positive Endospore-Forming Bacteria genetics isolation & purification growth & development MeSH
- Histones genetics isolation & purification MeSH
- rac GTP-Binding Proteins genetics isolation & purification MeSH
- Chromosome Segregation genetics MeSH
- Translocation, Genetic genetics MeSH
- Publication type
- Meeting Abstract MeSH
