Q84649300
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FTH_0069 is a previously uncharacterized strongly immunoreactive protein that has been proposed to be a novel virulence factor in Francisella tularensis. Here, the glycan structure modifying two C-terminal peptides of FTH_0069 was identified utilizing high resolution, high mass accuracy mass spectrometry, combined with in-source CID tandem MS experiments. The glycan observed at m/z 1156 was determined to be a hexasaccharide, consisting of two hexoses, three N-acetylhexosamines, and an unknown monosaccharide containing a phosphate group. The monosaccharide sequence of the glycan is tentatively proposed as X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide. The glycan is identical to that of DsbA glycoprotein, as well as to one of the multiple glycan structures modifying the type IV pilin PilA, suggesting a common biosynthetic pathway for the protein modification. Here, we demonstrate that the glycosylation of FTH_0069, DsbA, and PilA was affected in an isogenic mutant with a disrupted wbtDEF gene cluster encoding O-antigen synthesis and in a mutant with a deleted pglA gene encoding pilin oligosaccharyltransferase PglA. Based on our findings, we propose that PglA is involved in both pilin and general F. tularensis protein glycosylation, and we further suggest an inter-relationship between the O-antigen and the glycan synthesis in the early steps in their biosynthetic pathways.
- MeSH
- faktory virulence chemie genetika metabolismus MeSH
- Francisella tularensis genetika metabolismus patogenita MeSH
- glykosylace MeSH
- molekulární sekvence - údaje MeSH
- multigenová rodina MeSH
- mutace MeSH
- O-antigeny chemie genetika metabolismus MeSH
- proteiny fimbrií chemie genetika metabolismus MeSH
- sacharidové sekvence MeSH
- sekvence aminokyselin MeSH
- tandemová hmotnostní spektrometrie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- MeSH
- 2D gelová elektroforéza metody využití MeSH
- bakteriální proteiny analýza izolace a purifikace metabolismus MeSH
- chromatografie afinitní metody využití MeSH
- chromatografie MeSH
- financování organizované MeSH
- glykoproteiny analýza izolace a purifikace metabolismus MeSH
- glykosylace MeSH
- hmotnostní spektrometrie metody využití MeSH
- lidé MeSH
- polysacharidy chemie izolace a purifikace metabolismus MeSH
- western blotting metody využití MeSH
- Check Tag
- lidé MeSH
