• MeSH
• lidé MeSH
• počítačová rentgenová tomografie MeSH
• systémový lupus erythematodes komplikace   MeSH
• vaskulitida centrálního nervového systému při lupus erythematodes MeSH
• Check Tag
• lidé MeSH

PURPOSE: Osteoporosis is a severe health problem with social and economic impacts on society. The standard treatment consists of the systemic administration of drugs such as bisphosphonates, with alendronate (ALN) being one of the most common. Nevertheless, complications of systemic administration occur with this drug. Therefore, it is necessary to develop new strategies, such as local administration. METHODS: In this study, emulsion/dispersion scaffolds based on W/O emulsion of PCL and PF68 with ALN, containing hydroxyapatite (HA) nanoparticles as the dispersion phase were prepared using electrospinning. Scaffolds with different release kinetics were tested in vitro on the co-cultures of osteoblasts and osteoclast-like cells, isolated from adult osteoporotic and control rats. Cell viability, proliferation, ALP, TRAP and CA II activity were examined. A scaffold with a gradual release of ALN was tested in vivo in the bone defects of osteoporotic and control rats. RESULTS: The release kinetics were dependent on the scaffold composition and the used system of the poloxamers. The ALN was released from the scaffolds for more than 22 days. The behavior of cells cultured in vitro on scaffolds with different release kinetics was comparable. The difference was evident between cell co-cultures isolated from osteoporotic and control animals. The PCL/HA scaffold show slow degradation in vivo and residual scaffold limited new bone formation inside the defects. Nevertheless, the released ALN supported bone formation in the areas surrounding the residual scaffold. Interestingly, a positive effect of systemic administration of ALN was not proved. CONCLUSION: The prepared scaffolds enabled tunable control release of ALN. The effect of ALN was proved in vitro and in in vivo study supported peri-implant bone formation.

• MeSH
• alendronát * farmakologie   MeSH
• emulze farmakologie   MeSH
• hydroxyapatit farmakologie   MeSH
• inhibitory kostní resorpce * farmakologie   MeSH
• krysa rodu rattus MeSH
• osteoblasty MeSH
• osteogeneze MeSH
• osteoklasty MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nano-structure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (H-NCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated O-SiOx samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10°), while the H-NCD-coated samples were hydrophobic (contact angle >70°). NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.

• MeSH
• biokompatibilní potahované materiály farmakologie   MeSH
• buněčná adheze MeSH
• buněčná diferenciace MeSH
• diamant * MeSH
• lidé MeSH
• osteoblasty MeSH
• osteogeneze * MeSH
• proliferace buněk MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

V roce 2011 uplynulo 100 let od založení Kanceláře Slovníku jazyka českého - pracoviště, jež se stalo bezprostředním předchůdcem dnešního Ústavu pro jazyk český Akademie věd ČR. Publikace obsahuje příspěvky věnované tomuto výročí, které se zčásti vracejí do minulosti: do historie češtiny, české jazykovědné bohemistiky i do historie jazykovědného výzkumu v ÚJČ. Řada dalších příspěvků však ve svém úhrnu přináší reprezentativní pohled na současné bohemistické bádání. Informují o různých typech slovníků (např. etymologických, frazeologických, valenčních) a o jejich zpracovávání za pomoci moderních lexikografických nástrojů, o postupném zpřístupňování slovní zásoby staré češtiny, o elektronické přípravě edic starších českých textů. Přibližují práci na nových mluvnicích češtiny, které se neobejdou bez využívání korpusových dat. Věnují pozornost stylu projevů mluvených i psaných, komunikátů mediálních i elektronických. Připomínají rovněž završení dlouhodobých dialektologických výzkumů zpracováním Českého jazykového atlasu, rozvoj české onomastické školy nebo to, jak současní pracovníci v oblasti jazykové kultury nově promýšlejí klasický odkaz Pražské školy (např. pojmy norma a kodifikace).