A steady rise in the number of poly-sensitized patients has increased the demand for effective prophylactic strategies against multi-sensitivities. Probiotic bacteria have been successfully used in clinics and experimental models to prevent allergic mono-sensitization. In the present study, we have investigated whether probiotic bacteria could prevent poly-sensitization by imprinting on the immune system early in life. We used two recombinant variants of probiotic Escherichia coli Nissle 1917 (EcN): i) EcN expressing birch and grass pollen, poly-allergen chimera construct (EcN-Chim), and ii) an "empty" EcN without allergen expression (EcN-Ctrl). Conventional mice (CV) were treated with either EcN-Chim or EcN-Ctrl in the last week of the gestation and lactation period. Gnotobiotic mice received one oral dose of either EcN-Chim or EcN-Ctrl before mating. The offspring from both models underwent systemic allergic poly-sensitization and intranasal challenge with recombinant birch and grass pollen allergens (rBet v 1, rPhl p 1, and rPhl p 5). In the CV setting, the colonization of offspring via treatment of mothers reduced allergic airway inflammation (AAI) in offspring compared to poly-sensitized controls. Similarly, in a gnotobiotic model, AAI was reduced in EcN-Chim and EcN-Ctrl mono-colonized offspring. However, allergy prevention was more pronounced in the EcN-Ctrl mono-colonized offspring as compared to EcN-Chim. Mono-colonization with EcN-Ctrl was associated with a shift toward mixed Th1/Treg immune responses, increased expression of TLR2 and TLR4 in the lung, and maintained levels of zonulin-1 in lung epithelial cells as compared to GF poly-sensitized and EcN-Chim mono-colonized mice. This study is the first one to establish the model of allergic poly-sensitization in gnotobiotic mice. Using two different settings, gnotobiotic and conventional mice, we demonstrated that an early life intervention with the EcN without expressing an allergen is a powerful strategy to prevent poly-sensitization later in life.

• MeSH
• alergeny imunologie   MeSH
• alergie imunologie   MeSH
• antigeny rostlinné imunologie   MeSH
• bříza imunologie   MeSH
• epitelové buňky imunologie   MeSH
• Escherichia coli imunologie   MeSH
• gnotobiologické modely imunologie   MeSH
• homeostáza imunologie   MeSH
• imunitní systém imunologie   MeSH
• lipnicovité imunologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• probiotika aplikace a dávkování   MeSH
• pyl imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Specific allergen immunotherapy (AIT) is the only therapeutic method with positive impact on natural course of allergic disease - affecting clinical development (including the progression of rhinitis to asthma) and new sensitisations. The actual problem is the increasing number of patients manifesting poly-sensitivity in allergy skin tests and / or in specific IgE tests. Usually, AIT is not recommended in such individuals. The objective we are facing is that in many patients tested as poly-reactive, we have to distinguish in which cases it is a true polysensitization, and when it is due to cross-reactivity of specific IgE antibodies induced by panallergens. This may really determine when AIT may be an appropriate course of action. The article focuses on this problem in more detail, applying the long time Czech and Slovak experience with allergy testing and allergen immunotherapy.

• MeSH
• alergeny MeSH
• bronchiální astma MeSH
• desenzibilizace imunologická * MeSH
• kožní testy MeSH
• lidé MeSH
• zkřížené reakce MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Because of their irreplaceable lubricating properties mineral oils are used in many production branches. They are employed as lubricating and cutting oils (refrigerating emulsions). The dermotropic effect of mineral oils is caused by their varied chemical composition. Their common effect is irritating, photodynamic, keratoplastic, acnegenic, infectious and rarely cancerogenic. The allergogenie potency of mineral oils is weak, added substances being responsible for the allergic reactions. The authors recorded allergy to metalworking fluid with manifestation of occupational allergic contact eczema caused by the additive nonylphenolethoxylate ofthe nonoxynol-6 type. Nonylphenolethoxylates are surface active substances with many uses. Undiluted they may irritate the skin, there being few references on allergies. Epicutaneous tests revealed allergic reaction to metalworking fluid and to 2%, 1% and 0.1% aqueous solution of nonylphenolethoxylate. Tests in 10 control subjects were negative. Data on contact allergy to nonylphenolethoxylate were not published so far in this country.

• MeSH
• alergeny MeSH
• dospělí MeSH
• hormony kůry nadledvin terapeutické užití   MeSH
• kontaktní dermatitida diagnóza   etiologie   terapie   MeSH
• kožní testy MeSH
• lidé středního věku MeSH
• lidé MeSH
• minerální olej chemie   imunologie   škodlivé účinky   MeSH
• nemoci z povolání MeSH
• nonoxynol diagnostické užití   chemie   škodlivé účinky   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH