Tor barbs, Tor tor (Hamilton), a cyprinid fish collected from Barak Valley, Assam, India were examined for helminth parasites. A nematode species of the genus Rhabdochona Railliet, 1916 (Rhabdochonidae) was found in the intestine of the fish specimens. The morphology of these nematodes was characterised by the presence of a funnel shaped prostom, head with four submedian sublabia, four cephalic papillae, long and slender posterior glandular oesophagus in both sexes, presence of 14 caudal papillae and two unequal spicules with pointed proximal tips in male specimens, and prominent post-equatorial vulval position, posteriorly directed vagina, uterus with fully scattered eggs in female specimens, and a tail pointed with a small mucron in both sexes. This nematode species was subsequently identified as Rhabdochona hospeti Thapar, 1950, reported herein for the first time in the northeastern India. In addition to its morphological description based on light microscopy, partial 18S and 28S rDNA sequences were generated to assess phylogenetic relationships of R. hospeti with other species of Rhabdochona.

• MeSH
• Cyprinidae * parasitology   MeSH
• Phylogeny MeSH
• Nematoda * genetics   MeSH
• Fish Diseases * epidemiology   parasitology   MeSH
• Spiruroidea * MeSH
• Thelazioidea * MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• India MeSH

During a survey in agricultural fields of the sub-humid region of Meerut district, India, two strains of entomopathogenic nematodes, labelled CS31 and CS32, were isolated using the Galleria baiting technique. Based on morphological and morphometric studies, and molecular data, the nematodes were identified as Steinernema pakistanense, making this finding the first report of this species from India. For the first time, we performed a molecular and biochemical characterization of the bacterial symbiont of S. pakistanense. Furthermore, a co-phylogenetic analysis of the bacteria from the monophyletic clade containing a symbiont of S. pakistanense, together with their nematode hosts, was conducted, to test the degree of nematode-bacteria co-speciation. Both isolates were also tested in a laboratory assay for pathogenicity against two major pests, Helicoverpa armigera and Spodoptera litura. The morphology of the Indian isolates corresponds mainly to the original description, with the only difference being the absence of a mucron in first-generation females and missing epiptygmata in the second generation. The sequences of bacterial recA and gyrB genes have shown that the symbiont of S. pakistanense is closely related to Xenorhabdus indica, which is associated with some other nematodes from the 'bicornutum' group. Co-phylogenetic analysis has shown a remarkable congruence between the nematode and bacterial phylogenies, suggesting that, in some lineages within the Steinernema / Xenorhabdus complex, the nematodes and bacteria have undergone co-speciation. In the virulence assay, both strains caused a 100% mortality of both tested insects after 48 h, even at the lowest doses of 25 infective juveniles per insect, suggesting that S. pakistanense could be considered for use in the biocontrol of these organisms in India.

• MeSH
• Insect Control MeSH
• Phylogeny * MeSH
• Biological Coevolution * MeSH
• Larva growth & development   parasitology   MeSH
• Moths growth & development   parasitology   MeSH
• Symbiosis * MeSH
• Tylenchida anatomy & histology   classification   microbiology   pathogenicity   MeSH
• Virulence MeSH
• Xenorhabdus classification   physiology   MeSH
• Animals MeSH
• Check Tag
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• India MeSH

A new species of entomopathogenic nematode, Steinernema pwaniensis n. sp., belonging to the glaseri group, is described from Tanzania. The infective juveniles of S. pwaniensis n. sp. are characterized by a body length of 978 μm (808-1131), distance from anterior end to excretory pore of 86 μm (80-95) and a tail length of 87 μm (75-95). The ratios a, c and E% of S. pwaniensis n. sp. are substantially lower than those of all other African 'glaseri' group members. The first-generation males of S. pwaniensis n. sp. can be distinguished by a large spicule length of 92 μm (80-97) and by the absence of the caudal mucron, while second-generation males possess a short spine-like mucron. First-generation females have a peg-like tail tip bearing three spine-like projections. Second-generation females can be recognized by a slightly protruding vulva and well-developed post-anal swelling. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show that S. pwaniensis n. sp. forms a strongly supported monophyletic clade with two other East African species, S. ethiopiense and S. karii.

• MeSH
• Animal Structures anatomy & histology   MeSH
• Biometry MeSH
• DNA, Helminth chemistry   genetics   MeSH
• Phylogeny MeSH
• DNA, Ribosomal Spacer chemistry   genetics   MeSH
• Microscopy MeSH
• Rhabditida anatomy & histology   classification   genetics   isolation & purification   MeSH
• DNA, Ribosomal chemistry   genetics   MeSH
• RNA, Ribosomal, 28S genetics   MeSH
• Sequence Analysis, DNA MeSH
• Cluster Analysis MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Tanzania MeSH

Corimalia strejceki Schön& Skuhrovec,sp. nov. is described and illustrated from the Caucasus, Russia. The new species is compared with the allopatric C. aliena (Faust, 1890) differing in size, shape of scales, scale patch close to the scutellum, size of mucrones on the meso- and metatibiae, and also shape of the apex of the penis. Controversy over the number of segments in the antennae in the genus Corimalia is summarized. An annotated catalogue and key of all Corimaliini species in the Caucasus is provided.

• MeSH
• Animal Structures anatomy & histology   growth & development   MeSH
• Coleoptera anatomy & histology   classification   growth & development   MeSH
• Animal Distribution MeSH
• Organ Size MeSH
• Body Size MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Russia MeSH

A new steinernematid nematode, herein described as Steinernema poinari sp. n., was recovered by baiting soil samples with the last instar wax moth larvae Galleria mellonella in three localities of southwest Bohemia, Czech Republic. Morphological and molecular data confirmed this nematode as a new species which belongs to the affine/intermedium group. S. poinari sp. n. was characterized by male, female and infective juvenile morphological observations. Male spicules are robust with a pointed tip, SW% = 109 (98-123) and GS% = 70 (58-87). The tail terminus of the first generation have a papillated mucron, whereas in the second generation a short filamentous mucron sometimes develops. Females of the first generation have a moderately protruding vulva, postanal swelling absent or slightly developed. Second generation females have a long filamentous mucron on the tail tip. Infective juvenile have a body length of 768 µm, lateral field formula 2, 5, 6, 5, 2, 1, ratio D% of 46 (40-55), E% of 84 (76-95) and H% of 50 (43-56). The new species was characterized by sequences of ITS and D2D3 regions of ribosomal DNA. S. poinari sp. n. can also be separated from S. affine and S. intermedium by cross-breeding tests.

• MeSH
• Phylogeny MeSH
• Nematoda classification   genetics   physiology   ultrastructure   MeSH
• Insecta parasitology   MeSH
• DNA, Ribosomal Spacer genetics   MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic MeSH

A new species of parasitic nematode, Procamallanus (Procamallanus) pacificus n. sp., is described from the stomach of the Pacific shortfinned eel, Anguilla obscura (type host), and from the speckled longfin eel, Anguilla reinhardtii, from northern New Caledonia (Melanesia, South Pacific); from Anguilla sp. (cf. obscura) from the Fiji Islands (Melanesia, South Pacific); and from the giant mottled eel Anguilla marmorata from Futuna Island (Wallis and Futuna Islands, Polynesia). Although a total of 450 nematodes were collected, all specimens were females; this suggests either an extremely rare occurrence of males or parthenogenetic reproduction in this species. Procamallanus pacificus differs markedly from all congeners from fish hosts in possessing a greater number (4-9) of caudal mucrons in the female and by other morphological features. This parasite might become a serious pathogen of cultured eels in the region of the South Pacific. Batrachocamallanus Jackson and Tinsley, 1995 is considered a junior synonym of Procamallanus Baylis, 1923, to which 2 species are transferred as Procamallanus occidentalis (Jackson and Tinsley, 1995) n. comb. and Procamallanus siluranae (Jackson and Tinsley, 1995) n. comb. One third-stage larva of Procamallanus (Spirocamallanus) sp. was also recorded from Anguilla sp. (cf. obscura) from the Fiji Islands.

• MeSH
• Anguilla parasitology   MeSH
• Financing, Organized MeSH
• Nematoda classification   ultrastructure   MeSH
• Secernentea Infections parasitology   veterinary   MeSH
• Microscopy, Electron, Scanning veterinary   MeSH
• Fish Diseases parasitology   MeSH
• Animals MeSH
• Check Tag
• Female MeSH
• Animals MeSH
• Publication type
• Comparative Study MeSH