radioactive water springs Dotaz Zobrazit nápovědu
Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jáchymov spa. Jáchymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, four springs (Agricola, Behounek, C1, and Curie) have been used for spa purposes, that is for the treatment of nervous and rheumatic disorders by constantly produced radioactive gas radon (222 Rn) dissolved in the water. The radioactivity reaches 24 kBq/L. Using 16S rRNA gene sequence analysis, all four isolates were identified as members of the genus Kocuria, with two isolates designated 208 and 401 affiliated with Kocuria kristinae, while isolates 101 and 301 most likely with K. rhizophila. The content of fatty acids in polar lipids was determined by gas chromatography-mass spectrometry (GC-MS) and two PUFA, that is arachidonic and eicosapentaenoic, were identified. The position of double bonds was confirmed by GC-MS of 3-pyridylcarbinol (formerly picolinyl) esters. We assume that all four isolates of Kocuria produce PUFA to increase the stability of cell membranes, which may be impaired by the reaction of the reactive oxygen species. These can arise, for example, because of α radiation during 222 Rn decay.
Biomass-based decontamination methods are among the most interesting water treatment techniques. In this study, 2 cyanobacterial strains, Nostoc punctiforme A.S/S4 and Chroococcidiopsis thermalis S.M/S9, isolated from hot springs containing high concentrations of radium (226Ra), were studied to be associated with removal of radionuclides (238U and 226Ra) and heavy metal cadmium (Cd) from aqueous solutions. The adsorption equilibrium data was described by Langmuir and Freundlich isotherm models. Kinetic studies indicated that the sorption of 3 metals followed pseudo-second-order kinetics. Effects of biomass concentration, pH, contact time, and initial metal concentration on adsorption were also investigated. Fourier-transform infrared spectroscopy revealed active binding sites on the cyanobacterial biomass. The obtained maximum biosorption capacities were 630 mg g-1 and 37 kBq g-1 for 238U and 226Ra for N. punctiforme and 730 mg g-1 and 55 kBq g-1 for C. thermalis. These 2 strains showed maximum binding capacity 160 and 225 mg g-1, respectively for Cd adsorption. These results suggest that radioactivity resistant cyanobacteria could be employed as an efficient adsorbent for decontamination of multi-component, radioactive and industrial wastewater.
Lipids from microorganisms, and especially lipids from Archaea, are used as taxonomic markers. Unfortunately, knowledge is very limited due to the uncultivability of most Archaea, which greatly reduces the importance of the diversity of lipids and their ecological role. One possible solution is to use lipidomic analysis. Six radioactive sources were investigated, two of which are surface (Wettinquelle and Radonka) and four deep from the Svornost mine (Agricola, Behounek, C1, and Curie). A total of 15 core lipids and 82 intact polar lipids were identified from the membranes of microorganisms in six radioactive springs. Using shotgun lipidomics, typical Archaea lipids were identified in spring water, namely dialkyl glycerol tetraethers, archaeol, hydroxyarchaeol and dihydroxyarchaeol. Diverse groups of polar heads were formed in archaeal IPLs, whose polar heads are formed mainly by hexose, deoxyhexose, and phosphoglycerol. The analysis was performed using shotgun lipidomics and the structure of all molecular species was confirmed by tandem mass spectrometry. After acid hydrolysis, a mixture of polar compounds was obtained from the polar head. Further analysis by GC-MS confirmed that the carbohydrates were glucose and rhamnose. Analysis by HPLC-MS of diastereoisomers of 2-(polyhydroxyalkyl)-3-(O-tolylthiocarbamoyl)thiazolidine-4(R)-carboxylates revealed that both L-rhamnose and D-glucose are present in spring samples only in varying amounts. The glycoside composition depends on the type of spring, that is, Wettinquelle and Radonka springs are basically shallow groundwater, while the samples from the Svornost mine are deep groundwater and do not contain glycosides with rhamnose. This method enables quick screening for characteristic Archaea lipids, allowing decisions on whether to pursue further analyses, such as metagenomic analysis, to directly confirm the presence of Archaea.
The water quality research performed in the years 2003-2010 demonstrated an impact of the mine water pumped from the closed Olší uranium mine and discharged from the mine water treatment plant (MWTP) and groundwater from springs in the area on the water quality of the Hadůvka stream. The water ecosystems of the lower part of the Hadůvka stream are impacted mainly by water originated from the springs located in the stream valley and drained syenit subsoil, naturally rich in uranium. Those inflows caused a very high concentration of uranium measured in the water of the stream, which exceeds the given limit value. No negative impact on the water ecosystems of the receiving Bobrůvka River was found. This reduction of impact is caused by five times higher average daily flow rate of the Bobrůvka River in comparison with the Hadůvka stream, which results in a sufficient dilution of pollution from the Hadůvka.
- MeSH
- arsen analýza toxicita MeSH
- geologické sedimenty MeSH
- hornictví * MeSH
- kovy analýza toxicita MeSH
- kvalita vody MeSH
- luminiscence MeSH
- monitorování radiace MeSH
- mutageny analýza toxicita MeSH
- odpad tekutý - odstraňování MeSH
- Photobacterium účinky léků metabolismus MeSH
- radioaktivní látky znečišťující vodu analýza toxicita MeSH
- radioaktivní odpad MeSH
- řeky chemie MeSH
- Salmonella typhimurium účinky léků genetika MeSH
- testy genotoxicity MeSH
- uran analýza toxicita MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
6 stran ; 24 cm
- Klíčová slova
- Jáchymov (Karlovy Vary, Česko),
- MeSH
- horké prameny analýza MeSH
- kvalita vody MeSH
- radioaktivita MeSH
- radioaktivní znečištění vody MeSH
- radiochemie MeSH
- Geografické názvy
- Československo MeSH
- Konspekt
- Fyzikální chemie
- NLK Obory
- fyzika, biofyzika
- chemie, klinická chemie
- environmentální vědy
- NLK Publikační typ
- studie
- separáty
- MeSH
- lázně * MeSH
- minerální vody analýza MeSH
- monitorování radiace * metody přístrojové vybavení MeSH
- přírodní prameny analýza MeSH
- radioaktivní látky znečišťující vzduch analýza MeSH
- radioaktivní znečištění vody analýza MeSH
- radon * analýza MeSH
- znečištění vzduchu ve vnitřním prostředí analýza MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Maďarsko MeSH
- Polsko MeSH
- Slovenská republika MeSH
The activity concentrations of Beryllium-7 ((7)Be), a naturally occurring radioisotope produced in the atmosphere, were measured in leaves of birch-trees, above-ground parts of grass, soil and rainwater in the mountain massive Kralicky Sneznik (the northeast of the Czech Republic, altitude about 750 m) in the years of 2005, 2006 and 2007. Dried and ground samples of the plants and soils, and water samples from wet deposition were used to determine the (7)Be content using a semiconductor gamma spectrometer. The (7)Be values ranged from 147.0 to 279.6 Bq kg(-1), from 48.7 to 740.8 Bq kg(-1), from 2.1 to 8.7 Bq kg(-1), and from 0.6 to 1.9 Bq kg(-1) in birch-tree leaves, grass samples, soils, and rainwater, respectively. Insignificant inter-annual variations but significant increase in the (7)Be activity concentrations during the spring and summer months were observed in birch-tree leaves and grass samples. The seasonal variation of the (7)Be concentrations in grass samples correlated (R(2) = 0.4663 and 0.6489) with precipitation. No similar correlation was found for (7)Be in birch-tree leaves. Beryllium-7 content in birch-tree leaves and in aerial parts of grass was mainly caused by direct transport of (7)Be from wet deposition into aerial parts of the observed plants.
- MeSH
- beryllium metabolismus MeSH
- bříza metabolismus MeSH
- lipnicovité metabolismus MeSH
- listy rostlin metabolismus MeSH
- monitorování radiace MeSH
- radiační pozadí MeSH
- radioaktivní látky znečišťující vzduch metabolismus MeSH
- radionuklidy metabolismus MeSH
- roční období MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
