Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb), each year causing millions of deaths. In this article, we present the synthesis and biological evaluations of new potential antimycobacterial compounds containing a fragment of the first-line antitubercular drug pyrazinamide (PZA), coupled with methyl or ethyl esters of selected amino acids. The antimicrobial activity was evaluated on a variety of (myco)bacterial strains, including Mtb H37Ra, M. smegmatis, M. aurum, Staphylococcus aureus, Pseudomonas aeruginosa, and fungal strains, including Candida albicans and Aspergillus flavus. Emphasis was placed on the comparison of enantiomer activities. None of the synthesized compounds showed any significant activity against fungal strains, and their antibacterial activities were also low, the best minimum inhibitory concentration (MIC) value was 31.25 μM. However, several compounds presented high activity against Mtb. Overall, higher activity was seen in derivatives containing ʟ-amino acids. Similarly, the activity seems tied to the more lipophilic compounds. The most active derivative contained phenylglycine moiety (PC-ᴅ/ʟ-Pgl-Me, MIC < 1.95 μg/mL). All active compounds possessed low cytotoxicity and good selectivity towards Mtb. To the best of our knowledge, this is the first study comparing the activities of the ᴅ- and ʟ-amino acid derivatives of pyrazinamide as potential antimycobacterial compounds.
- MeSH
- aminokyseliny chemie farmakologie MeSH
- antibakteriální látky farmakologie MeSH
- antituberkulotika farmakologie MeSH
- Aspergillus flavus účinky léků MeSH
- buňky Hep G2 MeSH
- Candida albicans účinky léků MeSH
- chromatografie kapalinová MeSH
- koncentrace vodíkových iontů MeSH
- lidé MeSH
- magnetická rezonanční spektroskopie MeSH
- mikrobiální testy citlivosti MeSH
- Mycobacterium smegmatis účinky léků MeSH
- Mycobacterium tuberculosis účinky léků MeSH
- optická otáčivost MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- pyrazinamid chemie farmakologie MeSH
- Staphylococcus aureus účinky léků MeSH
- tuberkulóza farmakoterapie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Disseminated fusariosis is a life-threatening, invasive, opportunistic infection in immunocompromised patients, especially those with haematological malignancies. The prognosis is poor because these fungi are resistant to many of the available antifungal agents. We present a case of disseminated fusariosis caused by Fusarium proliferatum in a patient with severe aplastic anaemia complicated by a secondary infection of Aspergillus flavus, with a fatal outcome. We also review the documented Fusarium infections in immunocompromised hosts.
- MeSH
- antifungální látky farmakologie terapeutické užití MeSH
- aplastická anemie komplikace MeSH
- Aspergillus flavus účinky léků izolace a purifikace MeSH
- aspergilóza komplikace mikrobiologie MeSH
- fatální výsledek MeSH
- fusarióza komplikace diagnóza farmakoterapie mikrobiologie MeSH
- Fusarium účinky léků izolace a purifikace MeSH
- hostitel s imunodeficiencí * MeSH
- koinfekce MeSH
- lidé MeSH
- mladý dospělý MeSH
- oportunní infekce diagnóza farmakoterapie mikrobiologie MeSH
- triazoly terapeutické užití MeSH
- Check Tag
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
Cyanobacteria are photosynthetic prokaryotes found in a range of environments. They are infamous for the production of toxins, as well as bioactive compounds, which exhibit anticancer, antimicrobial and protease inhibition activities. Cyanobacteria produce a broad range of antifungals belonging to structural classes, such as peptides, polyketides and alkaloids. Here, we tested cyanobacteria from a wide variety of environments for antifungal activity. The potent antifungal macrolide scytophycin was detected in Anabaena sp. HAN21/1, Anabaena cf. cylindrica PH133, Nostoc sp. HAN11/1 and Scytonema sp. HAN3/2. To our knowledge, this is the first description of Anabaena strains that produce scytophycins. We detected antifungal glycolipopeptide hassallidin production in Anabaena spp. BIR JV1 and HAN7/1 and in Nostoc spp. 6sf Calc and CENA 219. These strains were isolated from brackish and freshwater samples collected in Brazil, the Czech Republic and Finland. In addition, three cyanobacterial strains, Fischerella sp. CENA 298, Scytonema hofmanni PCC 7110 and Nostoc sp. N107.3, produced unidentified antifungal compounds that warrant further characterization. Interestingly, all of the strains shown to produce antifungal compounds in this study belong to Nostocales or Stigonematales cyanobacterial orders.
- MeSH
- Anabaena chemie klasifikace růst a vývoj izolace a purifikace MeSH
- antifungální látky chemie izolace a purifikace farmakologie MeSH
- Aspergillus flavus účinky léků růst a vývoj MeSH
- Candida albicans účinky léků růst a vývoj MeSH
- cyklické peptidy chemie izolace a purifikace farmakologie MeSH
- druhová specificita MeSH
- fylogeneze MeSH
- glykolipidy chemie izolace a purifikace farmakologie MeSH
- lipopeptidy chemie izolace a purifikace farmakologie MeSH
- molekulární struktura MeSH
- molekulární typizace MeSH
- Nostoc chemie klasifikace růst a vývoj izolace a purifikace MeSH
- objevování léků * MeSH
- pyrany chemie izolace a purifikace farmakologie MeSH
- sinice chemie klasifikace růst a vývoj izolace a purifikace MeSH
- sladká voda mikrobiologie MeSH
- slané vody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Brazílie MeSH
- Česká republika MeSH
- Finsko MeSH
In the present study, genetic diversity and mycotoxin profiles of Aspergillus flavus isolated from air (indoors and outdoors), levels (surfaces), and soils of five hospitals in Southwest Iran were examined. From a total of 146 Aspergillus colonies, 63 isolates were finally identified as A. flavus by a combination of colony morphology, microscopic criteria, and mycotoxin profiles. No Aspergillus parasiticus was isolated from examined samples. Chromatographic analyses of A. flavus isolates cultured on yeast extract-sucrose broth by tip culture method showed that approximately 10% and 45% of the isolates were able to produce aflatoxin B(1) (AFB(1)) and cyclopiazonic acid (CPA), respectively. Around 40% of the isolates produced sclerotia on Czapek-Dox agar. The isolates were classified into four chemotypes based on the ability to produce AF and CPA that majority of them (55.5%) belonged to chemotype IV comprising non-mycotoxigenic isolates. Random amplified polymorphic DNA (RAPD) profiles generated by a combination of four selected primers were used to assess genetic relatedness of 16 selected toxigenic and non-toxigenic isolates. The resulting dendrogram demonstrated the formation of two separate clusters for the A. flavus comprised both mycotoxigenic and non-toxigenic isolates in a random distribution. The obtained results in this study showed that RAPD profiling is a promising and efficient tool to determine intra-specific genetic variation among A. flavus populations from hospital environments. A. flavus isolates, either toxigenic or non-toxigenic, should be considered as potential threats for hospitalized patients due to their obvious role in the etiology of nosocomial aspergillosis.
- MeSH
- Aspergillus flavus klasifikace genetika izolace a purifikace metabolismus MeSH
- fylogeneze MeSH
- mikrobiologie vzduchu MeSH
- molekulární sekvence - údaje MeSH
- mykotoxiny metabolismus MeSH
- nemocnice MeSH
- půdní mikrobiologie MeSH
- vybavení a zásoby nemocnice mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Írán MeSH
- MeSH
- Aspergillus flavus MeSH
- fyziologie výživy MeSH
- lidé MeSH
- nádory jater diagnóza etiologie terapie MeSH
- žloutenka MeSH
- Check Tag
- lidé MeSH
- MeSH
- amfotericin B * aplikace a dávkování terapeutické užití MeSH
- Aspergillus flavus * imunologie izolace a purifikace patogenita MeSH
- Aspergillus fumigatus imunologie izolace a purifikace patogenita MeSH
- Aspergillus nidulans imunologie izolace a purifikace patogenita MeSH
- aspergilóza alergická bronchopulmonální diagnóza etiologie terapie MeSH
- aspergilóza * diagnóza etiologie terapie MeSH
- dermatomykózy diagnóza komplikace terapie MeSH
- diagnostické techniky a postupy využití MeSH
- invazivní plicní aspergilóza diagnóza etiologie terapie MeSH
- itrakonazol aplikace a dávkování terapeutické užití MeSH
- klinické laboratorní techniky využití MeSH
- klinický obraz nemoci MeSH
- lidé MeSH
- nemoci imunitního systému imunologie komplikace mikrobiologie MeSH
- oportunní infekce komplikace prevence a kontrola terapie MeSH
- otomykóza diagnóza etiologie terapie MeSH
- plicní aspergilóza diagnóza etiologie klasifikace MeSH
- sinusitida diagnóza etiologie terapie MeSH
- statistika jako téma MeSH
- Check Tag
- lidé MeSH
Acta hygienica, epidemiologica et microbiologica, ISSN 0862-5956 1/2003
28 s. : il., tab. ; 21 cm
- MeSH
- Aspergillus flavus izolace a purifikace MeSH
- Aspergillus izolace a purifikace MeSH
- kontaminace potravin MeSH
- kultivační techniky metody normy MeSH
- mikrobiální testy citlivosti metody normy MeSH
- Publikační typ
- směrnice MeSH
- Konspekt
- Mikrobiologie
- NLK Obory
- mikrobiologie, lékařská mikrobiologie
- zemědělství a potravinářství
40 s. : il. ; 30 cm
